Active Recombinant Human PDE1C, GST-tagged

• Cat.No.: PDE1C-1612H
• Product Overview: Full-length recombinant human PDE1C was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Description: Cyclic nucleotide phosphodiesterases (PDEs) catalyze hydrolysis of the cyclic nucleotides cAMP and cGMP to the corresponding nucleoside 5-prime-monophosphates. Mammalian PDEs have been classified into several families based on their biochemical properties. Members of the PDE1 family, such as PDE1C, are calmodulin (see MIM 114180)-dependent PDEs (CaM-PDEs) that are stimulated by a calcium-calmodulin complex.
• Source: Sf9 insect cells
• Species: Human
• Tag: GST
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Bio-activity: 445 nmol/min/mg
• Molecular Mass: ~99 kDa
• Protein length: Full Length
• Purity: >90%
• Applications: PDE Assay
• Stability: 1 yr at -70oC from date of shipment.
• Storage: Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 ug/ul

Gene Information

• Gene Name: PDE1C phosphodiesterase 1C, calmodulin-dependent 70kDa [ Homo sapiens ]
• Official Symbol: PDE1C
• Synonyms: PDE1C; phosphodiesterase 1C, calmodulin-dependent 70kDa; phosphodiesterase 1C, calmodulin dependent (70kD); calcium/calmodulin-dependent 3,5-cyclic nucleotide phosphodiesterase 1C; Hcam3; hCam-3; cam-PDE 1C; Human 3,5 cyclic nucleotide phosphodiesterase (HSPDE1C1A)
• Gene ID: 5137
• mRNA Refseq: NM_005020
• Protein Refseq: NP_005011
• MIM: 602987
• UniProt ID: Q14123
• Chromosome Location: 7p14.3
• Pathway: Ca-dependent events; Calmodulin induced events; DAP12 interactions
• Function: calmodulin binding; calmodulin-dependent cyclic-nucleotide phosphodiesterase activity; metal ion binding