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PRMT5 Chemiluminescent Assay Kit
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Cat.No. : | Kit-1858 |
Product Overview : | PRMT5 is a histone methyltransferase that exhibits methylation activity toward H4-R3. |
Size : | 96 reactions |
Description : | The PRMT5 Direct Activity Chemiluminescent Assay Kit is designed to measure PRMT5 activity for screening and profiling applications. The key to the PRMT5 Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated R3 residue of Histone H4. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader. |
Applications : | Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications. |
Storage : | 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival. |
◆ Recombinant Protein
PRMT5-3004H | Recombinant Human PRMT5 Protein, MYC/DDK-tagged | +Inquiry |
Prmt5-5133M | Recombinant Mouse Prmt5 Protein, Myc/DDK-tagged | +Inquiry |
PRMT5-13419M | Recombinant Mouse PRMT5 Protein | +Inquiry |
PRMT5-32H | Recombinant Human PRMT5/WDR77 protein, FLAG/His-tagged | +Inquiry |
PRMT5-178H | Recombinant Human PRMT5/MEP50 complex Protein, Flag/His-tagged | +Inquiry |
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◆ Lysates
PRMT5-2577HCL | Recombinant Human PRMT5 cell lysate | +Inquiry |
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◆ Assay kits
Kit-1859 | PRMT5 Homogeneous Assay Kit | +Inquiry |
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