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PRMT5 Chemiluminescent Assay Kit
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| Cat.No. : | Kit-1858 |
| Product Overview : | PRMT5 is a histone methyltransferase that exhibits methylation activity toward H4-R3. |
| Size : | 96 reactions |
| Description : | The PRMT5 Direct Activity Chemiluminescent Assay Kit is designed to measure PRMT5 activity for screening and profiling applications. The key to the PRMT5 Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated R3 residue of Histone H4. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader. |
| Applications : | Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications. |
| Storage : | 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival. |
| Kit Components : | PRMT5/MEP50: 10 µg; -80°C 20 µM S-adenosylmethionine*: 250 µl; -80°C Primary antibody 4: 100 µl; -80°C Secondary HRP-labeled antibody 2: 10 µl; -80°C4x HMT assay buffer 2: 3 ml; -20°C Blocking buffer: 50 ml; +4°C HRP chemiluminescent substrate A (translucent bottle): 6 ml; +4°C HRP chemiluminescent substrate B (brown bottle): 6 ml; +4°C 96-well plate precoated with histone substrate: 1 plate; +4°C* Decreasing S-adenosylmethionine concentration will make the assay more sensitive to the inhibitors. |
◆ Recombinant Protein
| PRMT5-3004H | Recombinant Human PRMT5 Protein, MYC/DDK-tagged | +Inquiry |
| PRMT5&MEP50-315H | Recombinant Human PRMT5&MEP50 protein, Flag/His-tagged | +Inquiry |
| PRMT5-6962H | Recombinant Human PRMT5, GST-tagged | +Inquiry |
| PRMT5-3614R | Recombinant Rhesus monkey PRMT5 Protein, His-tagged | +Inquiry |
| PRMT5-177H | Recombinant Human PRMT5 Protein, Flag-tagged | +Inquiry |
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◆ Lysates
| PRMT5-2577HCL | Recombinant Human PRMT5 cell lysate | +Inquiry |
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◆ Assay kits
| Kit-1859 | PRMT5 Homogeneous Assay Kit | +Inquiry |
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