Recombinant Full Length Human PRMT5 Protein, C-Flag-tagged

• Cat.No.: PRMT5-688HFL
• Product Overview: Recombinant Full Length Human PRMT5 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes an enzyme that belongs to the methyltransferase family. The encoded protein catalyzes the transfer of methyl groups to the amino acid arginine, in target proteins that include histones, transcriptional elongation factors and the tumor suppressor p53. This gene plays a role in several cellular processes, including transcriptional regulation, and the assembly of small nuclear ribonucleoproteins. A pseudogene of this gene has been defined on chromosome 4. Alternative splicing results in multiple transcript variants encoding different isoforms.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 72.5 kDa
• AA Sequence: MAAMAVGGAGGSRVSSGRDLNCVPE IADTLGAVAKQGFDFLCMPVFHPRF KREFIQEPAKNRPGPQTRSD LLLSGRDWNTLIVGKLSPWIRPDSK VEKIRRNSEAAMLQELNFGAYLGLP AFLLPLNQEDNTNLARVLTN HIHTGHHSSMFWMRVPLVAPEDLRD DIIENAPTTHTEEYSGEEKTWMWWH NFRTLCDYSKRIAVALEIGA DLPSNHVIDRWLGEPIKAAILPTSI FLTNKKGFPVLSKMHQRLIFRLLKL EVQFIITGTNHHSEKEFCSY LQYLEYLSQNRPPPNAYELFAKGYE DYLQSPLQPLMDNLESQTYEVFEKD PIKYSQYQQAIYKCLLDRVP EEEKDTNVQVLMVLGAGRGPLVNAS LRAAKQADRRIKLYAVEKNPNAVVT LENWQFEEWGSQVTVVSSDM REWVAPEKADIIVSELLGSFADNEL SPECLDGAQHFLKDDGVSIPGEYTS FLAPISSSKLYNEVRACREK DRDPEAQFEMPYVVRLHNFHQLSAP QPCFTFSHPNRDPMIDNNRYCTLEF PVEVNTVLHGFAGYFETVLY QDITLSIRPETHSPGMFSWFPILFP IKQPITVREGQTICVRFWRCSNSKK VWYEWAVTAPVCSAIHNPTG
  RSYTIGLTRTRPLEQKLISEEDLAA NDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Stem cell - Pluripotency

Gene Information

• Gene Name: PRMT5 protein arginine methyltransferase 5 [ Homo sapiens (human) ]
• Official Symbol: PRMT5
• Synonyms: HSL7; JBP1; SKB1; IBP72; SKB1Hs; HRMT1L5
• Gene ID: 10419
• mRNA Refseq: NM_006109.5
• Protein Refseq: NP_006100.2
• MIM: 604045
• UniProt ID: O14744

Reviews

02/18/2023

Great performance in ELISA.

11/01/2018

Its purity and functionality ensure precise and reproducible results, enabling me to achieve my research objectives with confidence.

10/30/2016

The PRMT5 protein demonstrates exceptional quality, making it an ideal candidate to fulfill my experimental requirements.

Q&As

Are there any ongoing clinical trials targeting PRMT5?

Yes, there are ongoing clinical trials investigating the efficacy of PRMT5 inhibitors in various cancer types.

How does PRMT5 affect cellular differentiation?

PRMT5 influences cellular differentiation by regulating the expression of genes involved in the process.

Can PRMT5 inhibitors be used in combination therapies?

Yes, there is interest in exploring combination therapies involving PRMT5 inhibitors with other anticancer agents to enhance treatment efficacy.

How does PRMT5 contribute to epigenetic regulation?

PRMT5 methylates histone proteins, leading to changes in chromatin structure and gene expression, thus playing a crucial role in epigenetic regulation.

How does PRMT5 influence neurodegenerative diseases?

PRMT5 has been implicated in neurodegenerative diseases, and understanding its role may provide insights into developing therapies for conditions like Alzheimer's and Parkinson's disease.