PRMT5
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Official Full Name
protein arginine methyltransferase 5 -
Overview
Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles. Methylates SUPT5H. Mono- and dimethylates arginine residues of myelin basic protein (MBP) in vitro. Plays a role in the assembly of snRNP core particles. May play a role in cytokine-activated transduction pathways. Negatively regulates cyclin E1 promoter activity and cellular proliferation. May regulate the SUPT5H transcriptional elongation properties. May be part of a pathway that is connected to a chloride current, possibly through cytoskeletal rearrangement. Methylates histone H2A and H4 'Arg-3' during germ cell development. Methylates histone H3 'Arg-8', which may repress transcription. Methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage. Methylates RPS10. -
Synonyms
PRMT5;protein arginine methyltransferase 5;JBP1;SKB1;IBP72;SKB1Hs;HRMT1L5;protein arginine N-methyltransferase 5;SKB1 homolog;jak-binding protein 1;72 kDa ICln-binding protein;HMT1 hnRNP methyltransferase-like 5;shk1 kinase-binding protein 1 homolog;histone-arginine N-methyltransferase PRMT5
Recombinant Proteins
- Human
- Mouse
- Zebrafish
- Rhesus macaque
- E.coli
- HEK293
- Mammalian Cells
- Sf9 Cells
- Insect Cells
- Human Cells
- GST
- Flag
- His
- Non
- Avi
- Fc
- DDK
- Myc
Background
What is prmt5 protein?
PRMT5 (Protein Arginine Methyltransferase 5) is an enzyme that participates in the process of protein arginine methylation. It specifically catalyzes the transfer of methyl groups from S-adenosylmethionine (SAM) to arginine residues in target proteins. PRMT5 is involved in various cellular processes including gene expression regulation, RNA splicing, DNA damage response, and signal transduction pathways. It plays a crucial role in the development and function of cells, and its dysregulation has been associated with several diseases, including cancer.
What is the function of prmt5 protein?
Gene expression regulation: It methylates histones and transcription factors, influencing chromatin structure and transcription.
Epigenetic gene silencing: PRMT5 mediates the formation of symmetric dimethyl arginine marks on histones involved in gene repression.
Alternative splicing: It methylates RNA-binding proteins like Sm proteins to regulate spliceosome activity and RNA splicing.
mRNA processing and transport: PRMT5 influences messenger RNA metabolism by modifying proteins of this pathway.
Cell signaling: It methylates non-histone proteins in key signaling cascades like Wnt, MAPK, PI3K-Akt etc.
Prmt5 related signaling pathway
Wnt/β-catenin pathway: PRMT5 methylates components of this pathway like adenomatous polyposis coli (APC) protein.
MAPK signaling pathway: It regulates the MAPK pathway by methylating Ras, Raf and MEK kinases.
PI3K/Akt signaling: PRMT5 methylation influences PI3K/Akt cascade involved in growth, proliferation and metabolism.
Hippo signaling pathway: Modulates the Hippo pathway by methylating key tumor suppressors like Lats2.
PRMT5 Related Diseases
Cancer: Overexpression linked to various cancers like leukemia, lung, breast, prostate cancers. Promotes oncogenesis.
Viral infections: Required for replication of certain viruses like HIV, influenza and herpes simplex viruses.
Immunodeficiency: PRMT5 deficiency impairs immune cell development and function, predisposing to infections.
Metabolic syndrome: Dysregulation associated with insulin resistance, obesity, diabetes and fatty liver disease.
Biomedical Application of PRMT5 Protein
- Cancer therapy: PRMT5 inhibitors show promise against certain hematological and solid tumors.
- Anti-viral drugs: Targeting PRMT5-virus protein interactions could yield new antiviral strategies.
- Immunomodulation: Modulating its activity may benefit immunodeficiencies and autoimmune disorders.
- Anti-fibrotic therapy: Attenuating TGF-β signaling via PRMT5 inhibition offers organ fibrosis treatment.
Case Study
(Hong-feng Yuan, 2022)
Fig2. Succinylation levels of exogenous PRMT5 were tested by immunoprecipitation (IP) analysis in HepG2 cells treated with NAM and TSA as indicated.
(Antja-Voy Hartley , 2020)
Fig3. S15 phosphorylation is critical for the methyltransferase activity of PRMT5. AlphaLISA assay was conducted by using biotinylated histone H4 as a PRMT5 substrate. Graph shows detection of specific methyltransferase activity of WT-PRMT5 (WT) or S15A-PRMT5 (S15A) mutant enzymes purified from HEK293 cells in the presence or absence of 10 ng/mL of IL-1β. E444D-PRMT5 (E444D) was used as an enzymatic dead mutant control. S-adenosyl methionine (SAM) was used as the methyl donor for the reaction. The data represent the means ± standard deviation (S.D.) for three independent experiments.
Quality Guarantee
High Purity
Fig1. SDS-PAGE (Cat. No.: PRMT5-4447H)
Involved Pathway
PRMT5 involved in several pathways and played different roles in them. We selected most pathways PRMT5 participated on our site, such as Chromatin modifying enzymes,Chromatin organization,E2F transcription factor network, which may be useful for your reference. Also, other proteins which involved in the same pathway with PRMT5 were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.
| Pathway Name | Pathway Related Protein |
|---|---|
| RNA transport | EIF4BB,EIF2B4,SUMO3,SUMO3A,EIF5B,SUMO3B,EIF4EBP3,EEF1A1L1,STRAP,SIP1 |
| Metabolism of non-coding RNA | PEAK1,WDR77,DDX20,CLNS1A,SNUPN |
| RMTs methylate histone arginines | SMARCD3B,SMARCB1,ARID1A,SMARCC1B,SMARCA2,SMARCC2,SMARCC1,SMARCD3,SMARCD1,SMARCE1 |
| Chromatin organization | CSRP2BP,KDM5C,CHD4,ACTL6B,PHF2,DMAP1,KANSL2,CHD4A,KAT7,KDM1B |
| Chromatin modifying enzymes | PADI3,BRMS1,DNMT3A,TADA2A,MORF4L2,SMARCD3,KAT5,TADA1,DR1,KAT6A |
| Signaling events mediated by HDAC Class I | MXD1,FKBP3,SIRT4,TFCP2,SIRT5,NR2C1,GATA1,WDR77,SIRT7,GATA2 |
| E2F transcription factor network | RBBP8,E2F6,HIC1,CDKN2C,SMARCA2,CES1,RYBP,CES3,CEBPA,TRIM28 |
| Gene Expression | BRF1B,HELZ2,ZNF582,AEBP2,ZNF554,RDBP,ZNF446,HIPK1,LAGE3,CSTF1 |
Protein Function
PRMT5 has several biochemical functions, for example, core promoter sequence-specific DNA binding,histone-arginine N-methyltransferase activity,methyltransferase activity. Some of the functions are cooperated with other proteins, some of the functions could acted by PRMT5 itself. We selected most functions PRMT5 had, and list some proteins which have the same functions with PRMT5. You can find most of the proteins on our site.
| Function | Related Protein |
|---|---|
| protein-arginine N-methyltransferase activity | PRDM12,METTL21D,PRMT8B,RNMTL1A,PRDM11,METTL21A,NDUFAF5,CARM1,METTL23,TRMT10B |
| transcription corepressor activity | SIRT6,TAF9B,MXD4,ZBTB32,CBX4,LMCD1,SMYD1,SAP18,GMNN,NSD1 |
| protein binding | SKA1,CPNE7,NFS1,VAV3,NCALD,DALRD3,ZNF707,KIF6,FAM167A,CSNK1E |
| core promoter sequence-specific DNA binding | NFYA,MYOCD,SOHLH1,TRP53,NRIP1,PPARG,SOX9,SP1,ZFP932,ZFP750 |
| protein heterodimerization activity | HIST1H2AI,BHLHE40,CUL3,H2AFB2,BNIP3,HIST3H2BB,HIST1H47,LTC4S,CHRNB4,DR1 |
| protein complex binding | VPS28,MTHFR,IGBP1,AP2A1,AKAP5,GRIN2A,WRN,BAG3,WASF1,PAFAH1B1 |
| methyltransferase activity | THUMPD3,SMYD2A,PRMT1,FTSJD1,THUMPD2,RG9MTD1,TRMT13,DNMT3AA,DNMT5,C2orf56 |
| ribonucleoprotein complex binding | WDR12,SRP54,PRPF31,BOP1,SECISBP2,PES1,RNF135,CD2BP2,CKAP5,PQBP1 |
| protein-arginine omega-N symmetric methyltransferase activity | PRMT7 |
Interacting Protein
PRMT5 has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with PRMT5 here. Most of them are supplied by our site. Hope this information will be useful for your research of PRMT5.
WDR77;YWHAZ;E2F1;CLNS1A
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References
- Uzdensky, A; Demyanenko, S; et al. Expression of proteins involved in epigenetic regulation in human cutaneous melanoma and peritumoral skin. TUMOR BIOLOGY 35:8225-8233(2014).
- McLaughlin, N; Wang, FL; et al. In situ histone landscape of nephrogenesis. EPIGENETICS 9:222-235(2014).
