Creative BioMart to Present at
                        BIO-Europe Spring Creative BioMart to Present at IMMUNOLOGY2024™|May 3-7, 2024|Booth #512

Recombinant Active Human IL18 Protein, His-tagged(C-ter)

Cat.No. : IL18-154H
Product Overview : Recombinant Active Human IL18 Protein with His tag (C-ter) was expressed in E. coli.
  • Specification
  • Gene Information
  • Related Products
  • Download
Description : The protein encoded by this gene is a proinflammatory cytokine that augments natural killer cell activity in spleen cells, and stimulates interferon gamma production in T-helper type I cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2011]
Source : E. coli
Species : Human
Tag : His(C-ter)
Form : Powder
Bio-activity : Determined by its ability to induce IFN gamma secretion in KG-1 cells. The ED50 for this effect is < 5 ng/mL.
AA Sequence : MYFGKLESKLSVIRNLNDQVLFIDQ GNRPLFEDMTDSDCRDNAPRTIFII SMYKDSQPRGMAVTISVKCEKISTL SCENKIISFKEMNPPDNIKDTKSDI IFFQRSVPGHDNKMQFESSSYEGYF LACEKERDLFKLILKKEDELGDRSI MFTVQNED
Endotoxin : Endotoxin level is less than 0.1 EU/μg of the protein, as determined by the LAL test.
Purity : > 98% (by SDS-PAGE)
Applications : SDS-PAGE
Notes : For laboratory research only, not for drug, diagnostic or other use.
Storage : Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
Storage Buffer : PBS (pH 8.0)
Reconstitution : It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely.
Gene Name : IL18 interleukin 18 (interferon-gamma-inducing factor) [ Homo sapiens ]
Official Symbol : IL18
Synonyms : IL18; interleukin 18 (interferon-gamma-inducing factor); interleukin-18; IGIF; IL 1g; IL 18; IL1F4; IL-1 gamma; iboctadekin; interleukin-1 gamma; IFN-gamma-inducing factor; IL-18; IL-1g; MGC12320;
Gene ID : 3606
mRNA Refseq : NM_001243211
Protein Refseq : NP_001230140
MIM : 600953
UniProt ID : Q14116

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Inquiry

  • Q&As
  • Reviews

Q&As (10)

Ask a question
How do researchers experimentally investigate the crosstalk between IL18 and other immune signaling pathways, such as Toll-like receptor signaling? 10/28/2022

Co-immunoprecipitation, co-localization studies, and pathway inhibition assays decipher IL18's interactions with signaling molecules and pathways.

What assays and methodologies are employed to evaluate the potential therapeutic interventions targeting IL18 in autoimmune diseases and chronic inflammation? 03/23/2022

Therapeutic antibody trials, in vitro drug screens, and disease model assessments assess IL18-targeted interventions in autoimmune diseases.

How are animal models and in vivo imaging techniques used to understand the kinetics of IL18-mediated immune responses and its tissue-specific effects? 01/10/2022

Xenograft models, intravital imaging, and tissue-specific knockout approaches unravel IL18's kinetics and effects within complex in vivo environments.

Can you elaborate on the techniques used to assess the impact of IL18 on immune cell activation, migration, and effector functions? 06/11/2021

Flow cytometry, chemotaxis assays, and functional cell assays examine IL18's impact on immune cell activation, migration, and effector functions.

Can you explain the experimental strategies used to analyze the interactions between IL18 and its receptor complex, as well as the downstream signaling events triggered by receptor engagement? 01/12/2021

Surface plasmon resonance, mutational studies, and phosphoproteomics illuminate IL18's receptor interactions and downstream signal transduction pathways.

How are cellular and molecular techniques employed to investigate IL18's role in regulating the balance between Th1 and Th2 immune responses? 08/01/2020

Immunophenotyping, cytokine profiling, and gene expression analyses elucidate IL18's influence on Th cell polarization and immune balance.

What experimental approaches are utilized to study the genetic and epigenetic regulation of IL18 expression in response to different stimuli? 04/29/2020

ChIP assays, promoter analyses, and epigenetic profiling uncover the regulatory mechanisms underlying IL18 gene expression dynamics.

What methods are used to dissect the mechanisms through which IL18 contributes to the pathogenesis of inflammatory diseases and its interactions with other proinflammatory molecules? 12/15/2018

Disease models, knockout mice, and co-stimulation studies explore IL18's involvement in inflammatory conditions and its interplay with other proinflammatory mediators.

How do researchers investigate the role of IL18 in modulating the tumor microenvironment and its potential implications for cancer immunotherapy? 11/05/2018

Tumor models, immune profiling, and immunotherapeutic studies investigate IL18's modulation of the tumor microenvironment and its implications for cancer therapy.

Could you explain the experimental strategies used to characterize the inflammasome activation triggered by IL18 and its downstream effects on cytokine production? 04/20/2018

Caspase assays, cytokine ELISAs, and microscopy techniques unveil the activation of inflammasomes and IL18's contribution to pyroptosis and cytokine storms.

Customer Reviews (3)

Write a review
Reviews
10/15/2022

    Effective in inflammation models.

    08/22/2021

      High stability in serum.

      05/09/2019

        Suitable for ELISA.

        Ask a Question for All IL18 Products

        Required fields are marked with *

        My Review for All IL18 Products

        Required fields are marked with *

        0

        Inquiry Basket

        cartIcon
        logo

        FOLLOW US

        Terms and Conditions        Privacy Policy

        Copyright © 2024 Creative BioMart. All Rights Reserved.

        Contact Us

        • /

        Stay Updated on the Latest Bioscience Trends