Recombinant Human CCL21 Protein, His-tagged CCL21-2148H

Recombinant Human CCL21 Protein, His-tagged

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Recombinant Human CCL21 Protein, His-tagged

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Cat.No. : CCL21-2148H Optional Service: Optional requirements on this protein
Product Overview : Recombinant Human CCL21 Protein (Ser24-Pro134) with a N-His tag was expressed in E. coli.
Description : This antimicrobial gene is one of several CC cytokine genes clustered on the p-arm of chromosome 9. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. Similar to other chemokines the protein encoded by this gene inhibits hemopoiesis and stimulates chemotaxis. This protein is chemotactic in vitro for thymocytes and activated T cells, but not for B cells, macrophages, or neutrophils. The cytokine encoded by this gene may also play a role in mediating homing of lymphocytes to secondary lymphoid organs. It is a high affinity functional ligand for chemokine receptor 7 that is expressed on T and B lymphocytes and a known receptor for another member of the cytokine family (small inducible cytokine A19).
Source : E. coli
Species : Human
Tag : N-His
Form : Freeze-dried powder
Molecular Mass : Predicted Molecular Mass: 13.5 kDa
Accurate Molecular Mass: 18 kDa
Protein length : Ser24-Pro134
Endotoxin : <1.0 EU per 1µg (determined by the LAL method).
Purity : > 97%
Applications : Cell culture; Activity Assays.
Stability : The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage : Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
Storage Buffer : 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Reconstitution : Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
SDS-PAGE :

WB :

Bioactivity :

The chemotactic effect of SLC on Jurkat cells

The chemotactic effect of SLC on Jurkat cells.

Gene Name : CCL21 chemokine (C-C motif) ligand 21 [ Homo sapiens (human) ]
Official Symbol : CCL21
Synonyms : CCL21; chemokine (C-C motif) ligand 21; SCYA21, small inducible cytokine subfamily A (Cys Cys), member 21; C-C motif chemokine 21; 6Ckine; beta chemokine exodus 2; CKb9; ECL; Efficient Chemoattractant for Lymphocytes; exodus 2; secondary lymphoid tissue chemokine; SLC; TCA4; exodus-2; beta chemokine exodus-2; beta-chemokine exodus-2; small-inducible cytokine A21; secondary lymphoid-tissue chemokine; small inducible cytokine subfamily A (Cys-Cys), member 21; SCYA21; MGC34555;
Gene ID : 6366
mRNA Refseq : NM_002989
Protein Refseq : NP_002980
MIM : 602737
UniProt ID : O00585
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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