Species : |
Dog |
Source : |
E.coli |
Tag : |
Non |
Description : |
IL-3 is a hematopoieticgrowth factor that promotes the survival, differentiation and proliferationof committed progenitor cells of the megakaryocyte, granulocyte-macrophage,erythroid, eosinophil, basophil and mast cell lineages. Produced by T cells,mast cells and eosinophils, IL-3 enhances thrombopoieses, phagocytosis, andantibody-mediated cellular cytotoxicity. Its ability to activate monocytessuggests that IL-3 may have additional immunoregulatory roles. Many oftheIL-3 activities depend upon costimulation with other cytokines. IL-3 is aspecies-specific, variably glycosylated cytokine. |
Formulation : |
Lyophilized from a0.2 um filtered concentrated solution in PBS, pH 7.4. |
MolecularWeight : |
14.0 kDa |
Purity : |
>97% by SDS-PAGEand HPLC analyses |
Endotoxin Level : |
<0.1 ng/ug ofprotein |
Biological Activity : |
The ED50 asdetermined by the dose-dependent stimulation of the proliferation of humanTF-1 cells is less than 0.2 ng/ml, corresponding to a specific activity of5.0X10⁶ IU/mg. |
Reconstitution : |
Centrifuge vialpriorto opening. Reconstitute in sterile distilled water or aqueous buffercontaining 0.1%BSA to a concentration of 0.1-1.0 mg/ml. Stock solutionsshould be divided into working aliquots and stored at -800C. Furtherdilutions should be made in appropriate buffered solutions. |
Amino Acid Sequence : |
RPFSTDLPKQFTMINEIMEMLNKSPSPSE EPLDSNEKET LLEDTLLRPN LDVFLNASSK FHKNGLLIWN NLKEFLPLLP TPTPRGEPISIMENNWGDFQ RKLKKYLEALDNFLNFKNKP |
Storage : |
The lyophilizedprotein is stable at 2-4°C, but should be kept desiccated at -20°C for longterm storage. After reconstitution, the protein is stable for 1 week at2-4°C. For long term storage, aliquot and freeze at -80°C. Avoid repeatedfreeze-thaw cycles. |
OfficialSymbol : |
IL3 |