Recombinant Dog Interleukin 3 (colony-stimulating factor, multiple)

Name: Recombinant Dog Interleukin 3 (colony-stimulating factor, multiple)
SKU: IL3-362D

Species :	Dog
Source :	E.coli
Tag :	Non
Description :	IL-3 is a hematopoieticgrowth factor that promotes the survival, differentiation and proliferationof committed progenitor cells of the megakaryocyte, granulocyte-macrophage,erythroid, eosinophil, basophil and mast cell lineages. Produced by T cells,mast cells and eosinophils, IL-3 enhances thrombopoieses, phagocytosis, andantibody-mediated cellular cytotoxicity. Its ability to activate monocytessuggests that IL-3 may have additional immunoregulatory roles. Many oftheIL-3 activities depend upon costimulation with other cytokines. IL-3 is aspecies-specific, variably glycosylated cytokine.
Formulation :	Lyophilized from a0.2 um filtered concentrated solution in PBS, pH 7.4.
MolecularWeight :	14.0 kDa
Purity :	>97% by SDS-PAGEand HPLC analyses
Endotoxin Level :	<0.1 ng/ug ofprotein
Biological Activity :	The ED50 asdetermined by the dose-dependent stimulation of the proliferation of humanTF-1 cells is less than 0.2 ng/ml, corresponding to a specific activity of5.0X10⁶ IU/mg.
Reconstitution :	Centrifuge vialpriorto opening. Reconstitute in sterile distilled water or aqueous buffercontaining 0.1%BSA to a concentration of 0.1-1.0 mg/ml. Stock solutionsshould be divided into working aliquots and stored at -800C. Furtherdilutions should be made in appropriate buffered solutions.
Amino Acid Sequence :	RPFSTDLPKQFTMINEIMEMLNKSPSPSE EPLDSNEKET LLEDTLLRPN LDVFLNASSK FHKNGLLIWN NLKEFLPLLP TPTPRGEPISIMENNWGDFQ RKLKKYLEALDNFLNFKNKP
Storage :	The lyophilizedprotein is stable at 2-4°C, but should be kept desiccated at -20°C for longterm storage. After reconstitution, the protein is stable for 1 week at2-4°C. For long term storage, aliquot and freeze at -80°C. Avoid repeatedfreeze-thaw cycles.
OfficialSymbol :	IL3

Gene Name	IL3 interleukin 3(colony-stimulating factor, multiple) [ Canis lupus familiaris ]
Synonyms	IL3; interleukin 3(colony-stimulating factor, multiple); IL-3; MCGF; interleukin-3; mast cellgrowth factor; P-cell-stimulating factor; hematopoietic growth factor;multipotential colony-stimulating factor
Gene ID	481497
mRNA Refseq	NM_001013835
Protein Refseq	NP_001013857
UniProt ID	Q9BDX4
Pathway	Apoptosis; Asthma;Cytokine Signaling in Immune system; Cytokine-cytokine receptor interaction;Fc epsilon RI signaling pathway; Hematopoietic cell lineage; Immune System;Interleukin receptor SHC signaling; Interleukin-2 signaling; Interleukin-3, 5and GM-CSF signaling; Jak-STAT signaling pathway; Signaling by Interleukins