Recombinant Human Interleukin 3 (Colony-Stimulating Factor, Multiple)

• Cat.No.: IL3-275H
• Product Overview: Recombinant Human Interleukin-3 produced inSaccharomycescerevisiae is a single, glycosylated polypeptide chain containing 133 amino acids and having a molecular mass of 15000 Dalton. The recombinant human IL-3 migrates as a broad band between 15 and 22 kDa in SDS-PAGE due to post-translation modifications, in particular glycosylation.

Specification

• Cat. No.: IL3-275H
• Description: IL3 is a potent growth promoting cytokine. This cytokine is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders.
• Source: Saccharomyces cerevisiae.
• AA Sequence: A P M T Q T T P L K T S W V N C S N M I D E I I T H L K Q P P L P L L D F N N L N G E D Q D I L M E N N L R R P N L E A F N R A V K S L Q N A S A I E S I L K N L L P C L P L A T A A P T R H P I H I K D G D W N E F R R K L T F Y L K T L E N A Q A Q Q T T L S L A I F.
• Physical Appearance: Sterile Filtered white lyophilized (freeze-dried) powder.
• Formulation: Lyophilized from a concentrated (1mg/ml) solution in water containing 20mM Phosphate buffer pH-6.3.
• Purity: Greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Solubility: It is recommended to reconstitute the lyophilized Interleukin-3 in sterile 18Mω-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions.
• Biological Activity: The ED50 of IL-3 is typically 0.1- 0.4 ng/ml as measured in a cell proliferation assay using the human growth factor dependent TF-1 cell line.
• Stability: Lyophilized Interleukin-3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL3 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
• Tag: Non

Gene Information

• Gene Name: IL3 interleukin 3 (colony-stimulating factor, multiple) [ Homo sapiens ]
• Synonyms: IL3; interleukin 3 (colony-stimulating factor, multiple); IL-3; MCGF; MGC79398; MGC79399; MULTI-CSF; P-cell stimulating factor; hematopoietic growth factor; interleukin 3; mast-cell growth factor; multilineage-colony -stimulating factor; MULTI-CSF; Hematopoietic growth factor; P-cell-stimulating factor; Mast cell growth factor; Multipotential colony-stimulating factor
• Gene ID: 3562
• mRNA Refseq: NM_000588
• Protein Refseq: NP_000579
• MIM: 147740
• UniProt ID: P08700
• Chromosome Location: 5q31.1
• Pathway: Cytokine-cytokine receptor interaction; Jak-STAT signaling pathway; Asthma; Hematopoietic cell lineage; Fc epsilon RI signaling pathway
• Function: interleukin-3 receptor activity; cytokine activity; growth factor activity; protein binding

Reviews

06/20/2022

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03/08/2021

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12/19/2020

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Q&As

What is the difference between IL-3 and GM-CSF In vitro?

In vitro, IL-3 can induce differentiation and proliferation of myeloid lineage cells and is distinguished from GM-CSF by the relative increase in production of platelets, basophils, and mast cells.

What is the difference in clinical presentation of IL-3 compared to GM-CSF?

Although effective, IL-3 appears to present no therapeutic advantages compared to G-CSF and GM-CSF, and in direct comparison studies, IL-3 led to a considerably higher rate of adverse events than either of the other cytokines.

Since the structure of IL-3 is different between animals and humans, does it mean that il-3 is not necessary to maintain stable hematopoietic function?

mice deficient in both bc and IL-3 show the same phenotype as the bc-deficient mice, conclusively demonstrating that, for the most part, the GM-CSF/IL3/IL5 family is not essential for steady-state production of myeloid cells

How does the structure of IL-3 in animals differ from that in humans?

Unlike in humans, murine IL-3 has a second b chain receptor, bcIL3, which bears 91% homology to the bc chain.

Does loss of cytokine IL-3 affect normal granulocyte, monocyte, and basophil levels?

Knockout mice were also reported with surprisingly limited phenotypes. Despite the ability of the three cytokines to stimulate hematopoiesis, mice functionally deficient in all three cytokinesdisplay normal circulating levels of granulocytes, monocytes, and basophils.

IL-3 specific receptor subunit CD123 is overexpressed in many hematological malignancies,Is it overexpressed in normal tissues where inflammation occurs?

non-hematopoietic (e.g., epithelial cells, and fibroblasts) cell subsets can also produce il-3.

When is IL-3 activated by T cells?

Such as allergic inflammation and parasitic infestation.

Since basophils are the target and source of IL-3, can they regulate the secretion of IL-3 to treat allergic diseases?

IL-3 can regulate the survival of basophil granulocytes and cytokine production through autocrine, so as to achieve the purpose of treating allergic diseases.

T cell IL-3 expression is specifically regulated by which transcription factor?

Bhlhe40

Which hematopoietic and non-hematopoietic cell subsets express IL-3?

E.g., lymphocytes,epithelial cells, and fibroblasts

Are there any completed clinical trials on IL3?

Yes, there are some completed clinical trials on IL3 results, but the specific results need to refer to the relevant research papers.

What is the effect of IL3 on hematopoietic system?

This protein can promote stem cell proliferation and differentiation during hematopoietic process, thus increasing the production of different types of blood cells.

Are there any side effects of IL3?

In general, the IL3 has relatively few side effects, but some unpleasant symptoms such as fever, headache and nausea may occur.

IL3 associated with whether the immune adjustment?

IL3 can regulate the body's immune response and the function of immune cells, and plays an important role in immune regulation.

What is the solubility and stability of IL3?

IL3 generally have good solubility and stability, making them easier to handle during drug preparation and storage.

How is the research progress of IL3?

The study of IL3 is ongoing, including its application in the development of therapeutic drugs, as well as in combination therapy with other immune molecules.