Recombinant Human IL3 protein, His & GST-tagged

• Cat.No.: IL3-639H
• Product Overview: Recombinant Human IL3 aa. (Ala20~Phe152) fused with N-terminal His & GST tag was produced in E. coli cells.

Specification

• Description: The protein encoded by this gene is a potent growth promoting cytokine. This cytokine is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders.
• Source: E. coli
• Species: Human
• Tag: His & GST
• Form: Freeze-dried powder
• Molecular Mass: 45kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Ala20~Phe152
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 6.6.
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200μg/mL
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex

Gene Information

• Gene Name: IL3 interleukin 3 [ Homo sapiens (human) ]
• Official Symbol: IL3
• Synonyms: IL-3; MCGF; MULTI-CSF; P-cell stimulating factor; colony-stimulating factor, multiple; hematopoietic growth factor; mast-cell growth factor; multilineage-colony-stimulating factor; multipotential colony-stimulating factor; interleukin-3
• Gene ID: 3562
• mRNA Refseq: NM_000588.3
• Protein Refseq: NP_000579.2
• UniProt ID: P08700