Recombinant Full Length Human ALPL Protein, C-Flag-tagged

• Cat.No.: ALPL-192HFL
• Product Overview: Recombinant Full Length Human ALPL Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a member of the alkaline phosphatase family of proteins. There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature enzyme. This enzyme may play a role in bone mineralization. Mutations in this gene have been linked to hypophosphatasia, a disorder that is characterized by hypercalcemia and skeletal defects.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 55.5 kDa
• AA Sequence: MISPFLVLAIGTCLTNSLVPEKEKD PKYWRDQAQETLKYALELQKLNTNV AKNVIMFLGDGMGVSTVTAA RILKGQLHHNPGEETRLEMDKFPFV ALSKTYNTNAQVPDSAGTATAYLCG VKANEGTVGVSAATERSRCN TTQGNEVTSILRWAKDAGKSVGIVT TTRVNHATPSAAYAHSADRDWYSDN EMPPEALSQGCKDIAYQLMH NIRDIDVIMGGGRKYMYPKNKTDVE YESDEKARGTRLDGLDLVDTWKSFK PRYKHSHFIWNRTELLTLDP HNVDYLLGLFEPGDMQYELNRNNVT DPSLSEMVVVAIQILRKNPKGFFLL VEGGRIDHGHHEGKAKQALH EAVEMDRAIGQAGSLTSSEDTLTVV TADHSHVFTFGGYTPRGNSIFGLAP MLSDTDKKPFTAILYGNGPG YKVVGGERENVSMVDYAHNNYQAQS AVPLRHETHGGEDVAVFSKGPMAHL LHGVHEQNYVPHVMAYAACI
  GANLGHCAPASSAGSLAAGPLLLAL ALYPLSVLFTRTRPLEQKLISEEDL AANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome
• Protein Pathways: Folate biosynthesis, Metabolic pathways

Gene Information

• Gene Name: ALPL alkaline phosphatase, biomineralization associated [ Homo sapiens (human) ]
• Official Symbol: ALPL
• Synonyms: HOPS; HPPA; HPPC; HPPI; HPPO; TNAP; TNALP; APTNAP; TNSALP; AP-TNAP; TNS-ALP
• Gene ID: 249
• mRNA Refseq: NM_000478.6
• Protein Refseq: NP_000469.3
• MIM: 171760
• UniProt ID: P05186