Active Recombinant Human ALPL Protein (18-501aa), C-His-tagged

• Cat.No.: ALPL-01H
• Product Overview: Recombinant human Alkaline Phosphatase/ALPL (18-501aa), fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Specification

• Description: This gene encodes a member of the alkaline phosphatase family of proteins. There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature enzyme. This enzyme may play a role in bone mineralization. Mutations in this gene have been linked to hypophosphatasia, a disorder that is characterized by hypercalcemia and skeletal defects.
• Source: HEK293
• Species: Human
• Tag: C-His
• Form: Liquid
• Bio-activity: > 40,000 pmol/min/μg and is defined as the amount of enzyme that hydrolyze 1pmole of 4-Methylumbelliferyl phosphate to phosphate and 4-Methylumbelliferone per minute at pH 8.8 at 37 centigrade.
• Molecular Mass: 54.3 kDa (493aa)
• Protein Length: 18-501aa
• AA Sequence: LVPEKEKDPKYWRDQAQETLKYALE LQKLNTNVAKNVIMFLGDGMGVSTV TAARILKGQLHHNPGEETRLEMDKF PFVALSKTYNTNAQVPDSAGTATAY LCGVKANEGTVGVSAATERSRCNTT QGNEVTSILRWAKDAGKSVGIVTTT RVNHATPSAAYAHSADRDWYSDNEM PPEALSQGCKDIAYQLMHNIRDIDV IMGGGRKYMYPKNKTDVEYESDEKA RGTRLDGLDLVDTWKSFKPRYKHSH FIWNRTELLTLDPHNVDYLLGLFEP GDMQYELNRNNVTDPSLSEMVVVAI QILRKNPKGFFLLVEGGRIDHGHHE GKAKQALHEAVEMDRAIGQAGSLTS SEDTLTVVTADHSHVFTFGGYTPRG NSIFGLAPMLSDTDKKPFTAILYGN GPGYKVVGGERENVSMVDYAHNNYQ AQSAVPLRHETHGGEDVAVFSKGPM AHLLHGVHEQNYVPHVMAYAACIGA NLGHCAPAS
• Endotoxin: < 1 EU/μg of protein (determined by LAL method)
• Purity: > 95% by SDS-PAGE
• Applications: SDS-PAGE, Enzyme Activity
• Notes: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.
• Storage: Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/mL (determined by Absorbance at 280nm)
• Storage Buffer: Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Gene Information

• Gene Name: ALPL alkaline phosphatase, liver/bone/kidney [ Homo sapiens (human) ]
• Official Symbol: ALPL
• Synonyms: ALPL; alkaline phosphatase, liver/bone/kidney; HOPS; alkaline phosphatase, tissue-nonspecific isozyme; TNSALP; glycerophosphatase; tissue-nonspecific ALP; alkaline phosphomonoesterase; liver/bone/kidney-type alkaline phosphatase; alkaline phosphatase liver/bone/kidney isozyme; TNAP; APTNAP; AP-TNAP; FLJ40094; FLJ93059; MGC161443; MGC167935
• Gene ID: 249
• mRNA Refseq: NM_000478
• Protein Refseq: NP_000469
• MIM: 171760
• UniProt ID: P05186