Active Native Human Alkaline Phosphatase

• Cat.No.: ALPL-5324H
• Product Overview: Native human ALPL was purified from Human Liver.

Specification

• Description: Alkaline Phosphatase (ALP) is a hydrolase enzyme responsible for removing phosphate groups in the 5- and 3- positions from many types of molecules, including nucleotides, proteins, and alkaloids. In humans, alkaline phosphatase is present in all tissues throughout the entire body, but is particularly concentrated in liver, bile duct, kidney, bone, and the placenta. The optimal pH for the enzyme activity is pH=10 in standard conditions.
• Source: Human Liver
• Species: Human
• Form: Ammonium Sulfate Suspension
• Bio-activity: >25 U/mL
• Unit Definition: One unit will convert one micromole of p-nitrophenyl phosphate to p-nitrophenol and phosphate per minute at 37C in the presence of AMP (2-amino-2-methyl-1-propanol) at pH 10.35
• Storage: 2-8 °C
• Concentration: Typically >5 mg protein/mL

Gene Information

• Gene Name: ALPL alkaline phosphatase, liver/bone/kidney [ Homo sapiens ]
• Official Symbol: ALPL
• Synonyms: ALPL; alkaline phosphatase, liver/bone/kidney; HOPS; alkaline phosphatase, tissue-nonspecific isozyme; TNSALP; glycerophosphatase; tissue-nonspecific ALP; alkaline phosphomonoesterase; liver/bone/kidney-type alkaline phosphatase; alkaline phosphatase live
• Gene ID: 249
• mRNA Refseq: NM_000478
• Protein Refseq: NP_000469
• MIM: 171760
• UniProt ID: P05186
• Chromosome Location: 1p36.12
• Pathway: Endochondral Ossification, organism-specific biosystem; Folate biosynthesis, organism-specific biosystem; Folate biosynthesis, conserved biosystem; Metabolic pathways, organism-specific biosystem; TNF-alpha/NF-kB Signaling Pathway, organism-specific biosystem
• Function: alkaline phosphatase activity; hydrolase activity; metal ion binding