Recombinant Full Length Human PRC1 Protein, C-Flag-tagged

• Cat.No.: PRC1-1623HFL
• Product Overview: Recombinant Full Length Human PRC1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a protein that is involved in cytokinesis. The protein is present at high levels during the S and G2/M phases of mitosis but its levels drop dramatically when the cell exits mitosis and enters the G1 phase. It is located in the nucleus during interphase, becomes associated with mitotic spindles in a highly dynamic manner during mitosis, and localizes to the cell mid-body during cytokinesis. This protein has been shown to be a substrate of several cyclin-dependent kinases (CDKs). It is necessary for polarizing parallel microtubules and concentrating the factors responsible for contractile ring assembly. Alternative splicing results in multiple transcript variants.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 71.5 kDa
• AA Sequence: MRRSEVLAEESIVCLQKALNHLREI WELIGIPEDQRLQRTEVVKKHIKEL LDMMIAEEESLKERLIKSIS VCQKELNTLCSELHVEPFQEEGETT ILQLEKDLRTQVELMRKQKKERKQE LKLLQEQDQELCEILCMPHY DIDSASVPSLEELNQFRQHVTTLRE TKASRREEFVSIKRQIILCMEELDH TPDTSFERDVVCEDEDAFCL SLENIATLQKLLRQLEMQKSQNEAV CEGLRTQIRELWDRLQIPEEEREAV ATIMSGSKAKVRKALQLEVD RLEELKMQNMKKVIEAIRVELVQYW DQCFYSQEQRQAFAPFCAEDYTESL LQLHDAEIVRLKNYYEVHKE LFEGVQKWEETWRLFLEFERKASDP NRFTNRGGNLLKEEKQRAKLQKMLP KLEEELKARIELWEQEHSKA FMVNGQKFMEYVAEQWEMHRLEKER AKQERQLKNKKQTETEMLYGSAPRT PSKRRGLAPNTPGKARKLNT TTMSNATANSSIRPIFGGTVYHSPV SRLPPSGSKPVAASTCSGKKTPRTG RHGANKENLELNGSILSGGY
  PGSAPLQRNFSINSVASTYSEFAKD PSLSDSSTVGLQRELSKASKSDATS GILNSTNIQSTRTRPLEQKLISEED LAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.

Gene Information

• Gene Name: PRC1 protein regulator of cytokinesis 1 [ Homo sapiens (human) ]
• Official Symbol: PRC1
• Synonyms: ASE1
• Gene ID: 9055
• mRNA Refseq: NM_003981.4
• Protein Refseq: NP_003972.2
• MIM: 603484
• UniProt ID: O43663