Recombinant Human ABCB1 protein, His-tagged
Cat.No. : | ABCB1-5275H |
Product Overview : | Recombinant Human ABCB1 protein(P08183)(236-297aa), fused to N-terminal His tag, was expressed in E. coli. |
- Specification
- Gene Information
- Related Products
- Download
Source : | E. coli |
Species : | Human |
Tag : | His |
Form : | If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
Molecular Mass : | 10.8 kDa |
Protein length : | 236-297aa |
Purity : | Greater than 90% as determined by SDS-PAGE. |
Storage : | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
Reconstitution : | Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. |
Gene Name : | ABCB1 ATP-binding cassette, sub-family B (MDR/TAP), member 1 [ Homo sapiens ] |
Official Symbol : | ABCB1 |
Synonyms : | ABCB1; ATP-binding cassette, sub-family B (MDR/TAP), member 1; CLCS, colchicin sensitivity , MDR1, PGY1; multidrug resistance protein 1; ABC20; CD243; GP170; P gp; P-glycoprotein 1; colchicin sensitivity; doxorubicin resistance; CLCS; MDR1; P-GP; PGY1; |
Gene ID : | 5243 |
mRNA Refseq : | NM_000927 |
Protein Refseq : | NP_000918 |
MIM : | 171050 |
UniProt ID : | P08183 |
Products Types
◆ Recombinant Protein | ||
ABCB1-2132H | Recombinant Human ABCB1 protein(350-710aa), His-tagged | +Inquiry |
ABCB1-6R | Recombinant Rhesus Macaque ABCB1 Protein, His (Fc)-Avi-tagged | +Inquiry |
ABCB1-9C | Recombinant Cynomolgus Monkey ABCB1 Protein, His (Fc)-Avi-tagged | +Inquiry |
ABCB1-235H | Recombinant Human ABCB1 Protein, DDK-tagged | +Inquiry |
ABCB1-2591H | Recombinant Human ABCB1 protein(381-660 aa), C-His-tagged | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Inquiry
- Reviews
- Q&As
Q&As (11)
Ask a questionMany inhibitors can reverse ABCB1-mediated docetaxel resistance, different agents for different tumor, such as erastin in ovarian cancer.
In humans, ABCB1 genotyping in the treatment of depression rests on the assumption that genetic variations in ABCB1 explain individual differences in antidepressant response via their effects on P-gp expression at the blood-brain barrier.
Genetic variation in ABCB1 is associated with the pathogenesis of several dermatoses, including psoriasis, atopic dermatitis, melanoma, bullous pemphigoid, Behçet disease, and lichen planus.
Many agents could interact with ABCB1 including topical steroids, methotrexate, cyclosporine, azathioprine, antihistamines, antifungal agents, colchicine, tacrolimus, ivermectin, tetracycline, retinoid acids, and biologic agents.
There are large differences in the expression of P-glycoprotein in human liver, but this difference is not related to the C(3435)T and G(2677)T(A) polymorphisms in the ABCB1 gene.
Not only a nonpermissive chromatin structure (methylated) but either the absence of important transcriptional activators or the presence of repressors and co-repressors and the proteins like HDACs recruited by co-repressors can also inhibit gene transcription. The correlation between methylation and gene silencing for several genes are evidenced by researchers but many times there is absence of correlation observed for some genes too.
The convention is what is upstream of the downstream promoter is exon1a, and downstream of it, exon1b.
The human intestinal cell line Caco-2 has been reported to express,and cell lines such as MDCK and LLC-PK1 which have been transfected or transduced to overexpress ABCB1.
AMP-PNP, AMP-PCP, or ATP-gamma-S, but the required concentration will undoubtedly be quite high (mM).
Whether it is an artifact from nonspecific binding or a natural fragment of the target protein, the precise evaluation can be done by applying in-gel digestion followed by MS-based proteomic identification.
A related new study paper through recombinant mouse proteins has been published in the open access journal BioDiscovery, studying the second generation tyrosine kinase inhibitor (TKI) - dasatinib (DAS) and ATP binding (ABC) transport proteins ABCB1 and ABCG2 mechanism to assess whether these drug transporters may impair the therapeutic effect.
Ask a Question for All ABCB1 Products
Required fields are marked with *
My Review for All ABCB1 Products
Required fields are marked with *
Inquiry Basket