Recombinant Human ABCB1 protein, His-tagged

• Cat.No.: ABCB1-5275H
• Product Overview: Recombinant Human ABCB1 protein(P08183)(236-297aa), fused to N-terminal His tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
  If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
• Molecular Mass: 10.8 kDa
• Protein length: 236-297aa
• Purity: Greater than 90% as determined by SDS-PAGE.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%.

Gene Information

• Gene Name: ABCB1 ATP-binding cassette, sub-family B (MDR/TAP), member 1 [ Homo sapiens ]
• Official Symbol: ABCB1
• Synonyms: ABCB1; ATP-binding cassette, sub-family B (MDR/TAP), member 1; CLCS, colchicin sensitivity , MDR1, PGY1; multidrug resistance protein 1; ABC20; CD243; GP170; P gp; P-glycoprotein 1; colchicin sensitivity; doxorubicin resistance; CLCS; MDR1; P-GP; PGY1
• Gene ID: 5243
• mRNA Refseq: NM_000927
• Protein Refseq: NP_000918
• MIM: 171050
• UniProt ID: P08183

Q&As

What inhibitors could reverse the MDR of ABCB1?

Many inhibitors can reverse ABCB1-mediated docetaxel resistance, different agents for different tumor, such as erastin in ovarian cancer.

Is ABCB1 a supplement treatment for depression?

In humans, ABCB1 genotyping in the treatment of depression rests on the assumption that genetic variations in ABCB1 explain individual differences in antidepressant response via their effects on P-gp expression at the blood-brain barrier.

Does ABCB1 mutant could cause skin diseases?

Genetic variation in ABCB1 is associated with the pathogenesis of several dermatoses, including psoriasis, atopic dermatitis, melanoma, bullous pemphigoid, Behçet disease, and lichen planus.

How many agents could interact with ABCB1?

Many agents could interact with ABCB1 including topical steroids, methotrexate, cyclosporine, azathioprine, antihistamines, antifungal agents, colchicine, tacrolimus, ivermectin, tetracycline, retinoid acids, and biologic agents.

What's ther relationship between CT and GT(A) polymorphisms of ABCB1 gene and expression of human liver P-glycoprotein

There are large differences in the expression of P-glycoprotein in human liver, but this difference is not related to the C(3435)T and G(2677)T(A) polymorphisms in the ABCB1 gene.

How do cancer cells still have ABCB1 protein expression although CpG islands in its promoters are mostly methylated?

Not only a nonpermissive chromatin structure (methylated) but either the absence of important transcriptional activators or the presence of repressors and co-repressors and the proteins like HDACs recruited by co-repressors can also inhibit gene transcription. The correlation between methylation and gene silencing for several genes are evidenced by researchers but many times there is absence of correlation observed for some genes too.

What is the significance of dividing exon 1 into exon 1a and 1b at ABCB1 promoter region?

The convention is what is upstream of the downstream promoter is exon1a, and downstream of it, exon1b.

Which cell line would be best to use as a positive control for the expression of ABCB1 and ABCG2?

The human intestinal cell line Caco-2 has been reported to express,and cell lines such as MDCK and LLC-PK1 which have been transfected or transduced to overexpress ABCB1.

Are there any other compounds known to inhibit the ABCB1 ATPase apart from vanadate?

AMP-PNP, AMP-PCP, or ATP-gamma-S, but the required concentration will undoubtedly be quite high (mM).

What is it? - 100KDa band from ABCB1 blot with human lung fibroblasts

Whether it is an artifact from nonspecific binding or a natural fragment of the target protein, the precise evaluation can be done by applying in-gel digestion followed by MS-based proteomic identification.

How is the resistance of cancer cells affected by ABC-transporter?

A related new study paper through recombinant mouse proteins has been published in the open access journal BioDiscovery, studying the second generation tyrosine kinase inhibitor (TKI) - dasatinib (DAS) and ATP binding (ABC) transport proteins ABCB1 and ABCG2 mechanism to assess whether these drug transporters may impair the therapeutic effect.