Active Recombinant Human Acidic Fibroblast Growth Factor

• Cat.No.: FGF1-4H
• Product Overview: Human Acidic FGF is produced by genetically engineered yeast, and purified by heparin-Sepharose affinity chromatography.

Specification

• Description: Acidic FGF is a mitogen for cells of mesodermal and neuroectodermal origin, including fibroblasts, endothelial cells, astrocytes, neuroblasts, osteoblasts, and smooth muscle cells. This protein is composed of 140 amino acid residues, and has a 55% homology with basic FGF, including two conserved cysteine residues.?
• Source: yeast
• Species: Human
• Form: 10 mM Tris-HCl, 1.5 M NaCl, pH 7.0 Concentration: 0.50 mg protein/ml.
• Bio-activity: Human acidic FGF is mitogenic for a variety of normal cells in culture including fibroblasts, glial cells, vascular endothelial cells.
• Molecular Mass: 15.5 KDa
• Purity: Over 95% pure by HPLC analysis and SDS gel electrophoresis.
• Stability: Unstable without a carrier protein like gelatin or bovine serum albumin. It is recommended to add some of these proteins, prior to aliquoting, for longer storage.
• Storage: Store at -80°C. It is also possible to keep this protein at low pH (2-5) for a few days.

Gene Information

• Gene Name: FGF1 fibroblast growth factor 1 (acidic) [ Homo sapiens (human) ]
• Official Symbol: FGF1
• Synonyms: FGF1; fibroblast growth factor 1 (acidic); AFGF; ECGF; FGFA; ECGFA; ECGFB; HBGF1; GLIO703; FGF-alpha; ECGF-beta; Acidic fibroblast growth factor; OTTHUMP00000066031; heparin-binding growth factor 1; endothelial cell growth factor, beta; endothelial cell growth factor, alpha; Beta-endothelial cell growth factor; OTTHUMP00000066028; OTTHUMP00000174675; OTTHUMP00000066030
• Gene ID: 2246
• mRNA Refseq: NM_000800
• Protein Refseq: NP_000791
• MIM: 131220
• UniProt ID: P05230
• Chromosome Location: 5q31
• Pathway: MAPK signaling pathway; Melanoma; Pathways in cancer; Regulation of actin cytoskeleton
• Function: growth factor activity; heparin binding; protein binding