Active Recombinant Canine FGF1 protein

• Cat.No.: FGF1-159C
• Product Overview: Recombinant Canine FGF1 protein(XP_849274.1)(Phe16-Asp155) was expressed in E. coli.

Specification

• Species: Canine
• Source: E.coli
• Tag: Non
• Protein Length: Phe16-Asp155
• Form: Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Measured in a cell proliferation assay using Balb/c 3T3 mouse embryonic fibroblasts.The ED50 for this effect is typically 0.05-0.3 ng/ml.
• Molecular Mass: The recombinant canine FGF1 comprises 141 amino acids and has a predicted molecular mass of 16 kDa. The apparent molecular mass of the protein is approximately 16 kDa in SDS-PAGE under reducing conditions.
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.