Recombinant Human AIF1, His-tagged

• Cat.No.: AIF1-100H
• Product Overview: Total 155 aa; MW 17.7 kDa (calculated); N-terminal His-tag; 9 extra amino acids (highlighted).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: This gene is induced by cytokines and interferon. Its protein product is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma. The gene expresses three transcripts.
• Form: Filtered (0.4 μm) and lyophilized in 0.5 mg/mL in 5mM TRIS, 25mM NaCl, pH 7.5.
• Research topic: Energy metabolism and body weight regulation.
• Reconstitution: Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
• Applications: Western blotting
• Quality Control Test: BCA to determine quantity of the protein; SDS PAGE to determine purity of the protein
• Sequences of amino acids: MKHHHHHHAS QT­RDLQGGKA FGLLKAQQEE RLDEINKQFL DDPKYSSDED LPSKLEGFKE KYMEFDLNGN GDIDIMSLKR MLEKLGVPKT HLELKKLIGE VSSGSGETFS YPDFLRMMLG KRSAILKMIL MYEEKAREKE KPTGPPAKKA ISELP
• Storage: Store lyophilized protein at -20°C. Lyophilized protein remains stable until the expiry date when stored at -20°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -80°C for long term storage. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after one week at 4°C.

Gene Information

• Gene Name: AIF1 allograft inflammatory factor 1 [ Homo sapiens ]
• Official Symbol: AIF1
• Synonyms: AIF1; allograft inflammatory factor 1; IBA1; IRT1; AIF-1; IRT-1; protein G1; OTTHUMP00000029354; OTTHUMP00000029356; OTTHUMP00000029358; interferon gamma responsive transcript; ionized calcium-binding adapter molecule 1
• Gene ID: 199
• mRNA Refseq: NM_001623
• Protein Refseq: NP_001614
• MIM: 601833
• UniProt ID: P55008
• Chromosome Location: 6p21.3
• Function: actin filament binding; calcium ion binding