Recombinant Human BRD4 Protein (44-460), N-His tagged

• Cat.No.: BRD4-05H
• Product Overview: Human recombinant BRD4, tandem construct comprising bromodomains 1 and 2 (residues 44-460; Genbank Accession # NM_058243; MW = 49.7 kDa) expressed in E. coli with an N-terminal His-tag.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 44-460
• Description: The protein encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described.
• Molecular Mass: 49.7 kDa
• Purity: > 90% by SDS-PAGE
• Storage: At -80 centigrade, aliquot and snap-freeze after first use.
• Storage Buffer: Solution of purified recombinant protein in 50 mM Tris/HCl, pH 7.5, 500 mM NaCl, 1.0 mM TCEP, 10% glycerol (v/v)

Gene Information

• Gene Name: BRD4 bromodomain containing 4 [ Homo sapiens (human) ]
• Official Symbol: BRD4
• Synonyms: BRD4; bromodomain containing 4; CAP; MCAP; HUNK1; HUNKI; FSHRG4; bromodomain-containing protein 4; chromosome-associated protein; female sterile homeotic related gene 4; mitotic chromosome-associated protein; EC 3.1.1.31
• Gene ID: 23476
• mRNA Refseq: NM_058243
• Protein Refseq: NP_490597
• MIM: 608749
• UniProt ID: O60885