| Cat.No. : |
CA2-0237H |
| Product Overview : |
Human CA2 (NP_000058, 1 a.a. - 260 a.a.) full-length recombinant protein expressed in Escherichia coli. |
| Description : |
The protein encoded by this gene is one of several isozymes of carbonic anhydrase, which catalyzes reversible hydration of carbon dioxide. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2014] |
| Source : |
E. coli |
| Species : |
Human |
| Form : |
Liquid |
| Bio-activity : |
Specific activity is 50-70 nmoles/min/ug and was obtained by measuring the increase in the amount of p-nitropheno by its esterase activity. Specific activity is defined as the amount of p-nitrophenol that 1ug of enzyme can reduce at 25 centigrade for 1 minute. |
| Molecular Mass : |
29.2 kDa |
| AA Sequence : |
MSHHWGYGKH NGPEHWHKDF PIAKGERQSP VDIDTHTAKY DPSLKPLSVS YDQATSLRIL NNGHAFNVEF DDSQDKAVLK GGPLDGTYRL IQFHFHWGSL DGQGSEHTVD KKKYAAELHL VHWNTKYGDF GKAVQQPDGL AVLGIFLKVG SAKPGLQKVV DVLDSIKTKG KSADFTNFDP RGLLPESLDY WTYPGSLTTP PLLECVTWIV LKEPISVSSE QVLKFRKLNF NGEGEPEELM VDNWRPAQPL KNRQIKASFK |
| Purity : |
> 95% by SDS-PAGE |
| Applications : |
Functional Study
SDS-PAGE |
| Storage : |
Store at 4 centigrade for 1-2 weeks. For long term storage store at -20 centigrade or -80 centigrade. Aliquot to avoid repeated freezing and thawing. |
| Concentration : |
1 mg/mL |
| Storage Buffer : |
In 20 mM Tris-HCl, 50 mM NaCl, pH 8.0 (1 mM dithiothreitol, 10% glycerol) |
| Quality Control Testing : |
Loading 3 μg protein in 15% SDS-PAGE
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