Recombinant Human Carbonic Anhydrase I

• Cat.No.: CA1-179H
• Product Overview: Recombinant Human full length Carbonic Anhydrase I (Carbonate dehydratase I, CA-I, Carbonic anhydrase B) was cloned from human cDNA and expressed inE.coli.It consists of the human Carbonic Anhydrase I (Swiss prot accession: P00915). MW = 29.3 kDa.

Specification

• Cat. No.: CA1-179H
• Description: Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including cellular respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid.They show extensive diversity in tissue distribution and in their subcellular localization. CA1 is closely linked to CA2 and CA3 genes on chromosome 8, and it encodes a cytosolic protein which is found at the highest level in erythrocytes. Transcript variants of CA1 utilizing alternative polyA_sites have been described in literature.
• Source: E.Coli.
• Purity: > 95% by SDS-PAGE. The protein was observed as a single band migrating at molecular weight about 30 kDa.
• Specific Activity: >100 pmole/min/ug measured monitoring the esterase activity of the enzyme at 405nm with 1mM 4-nitrophenyl acetate in 12.5mM Tris, 75mM NaCl, pH 7.5.
• Supplied As: 2mg/ml in Tris buffer 20mM pH 7.5, NaCl 150mM. The concentration is calculated from the absorbance at 280nm (e280 = 44920 M-1 cm-1 ).
• Characteristics: Under the above described conditions, to avoid precipitation or protein dimerization, the product can be concentrated up to 1mM.
• Storage: -20ºC. The protein is stable at 4 ºC for weeks and at 25 ºC for at least several days. After initial defrost, aliquot product into individual tubes and refreeze at -20ºC. Avoid repeated freeze/defrost cycles.
• Tag: Non

Gene Information

• Gene Name: CA1 carbonic anhydrase I [ Homo sapiens ]
• Synonyms: CA1; carbonic anhydrase I; Car1; EC 4.2.1.1; Carbonic anhydrase 1; CA-I; Carbonic anhydrase B; CAB; carbonic dehydratase
• Gene ID: 759
• mRNA Refseq: NM_001128829
• Protein Refseq: NP_001122301
• MIM: 114800
• UniProt ID: P00915
• Chromosome Location: 8q21.2
• Pathway: Nitrogen metabolism
• Function: carbonate dehydratase activity; lyase activity; metal ion binding; zinc ion binding; carbonic dehydratase

Reviews

05/19/2022

Swift protein purification, expedites our research timelines.

04/23/2021

Efficient analysis, aids our understanding of protein function.

09/17/2020

Reliable protein assays, critical for our scientific investigations.

Q&As

How does CA1 protein's phosphorylation change during synaptic stimulation?

CA1 protein's phosphorylation changes with synaptic stimulation, impacting its function.

What impact do post-translational modifications have on CA1 protein's subcellular localization and synaptic function?

Post-translational modifications can alter CA1 protein's subcellular localization and synaptic function.

How do epigenetic factors influence CA1 protein's function in memory formation?

Epigenetic factors influence CA1 protein's function in memory formation via modifications.

What is CA1 protein's specific role in regulating memory processes?

CA1 protein plays a specific role in memory processes, aiding memory formation.

What is CA1 protein's specific role in regulating synaptic plasticity?

CA1 protein regulates synaptic plasticity by modulating synaptic strength.

How do genetic factors regulate CA1 protein expression?

Genetic factors regulate CA1 protein expression, affecting its levels.

Can we develop a high-resolution imaging technique to monitor CA1 protein dynamics within dendritic spines?

Developing high-resolution imaging techniques for monitoring CA1 protein dynamics is feasible.