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Recombinant Human Carbonic Anhydrase I, His-tagged

Cat.No. : CA1-2505H
Product Overview : Recombinant human Carbonic anhydrase1, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography. MW: 31.0 kDa.
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Description : CA1 is a zinc metalloenzyme that catalyses reversible hydration of CO2 (CO2 + H2O<->HCO3- + H+). This protein is fundamental to many biological processes such as cellular respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, salvia, and gastric acid. It is most abundant in erythrocytes and is a very early marker for erythroid differentiation.
Source : E. coli
Species : Human
Tag : His
Form : Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol.
Molecular Mass : 31.0 kDa (281aa), confirmed by MALDI-TOF
Protein length : 1-261aa
AA Sequence : MGSSHHHHHH SSGLVPRGSH MASPDWGYDD KNGPEQWSKL YPIANGNNQS PVDIKTSETK HDTSLKPISV SYNPATAKEI INVGHSFHVN FEDNDNRSVL KGGPFSDSYR LFQFHFHWGS TNEHGSEHTV DGVKYSAELH VAHWNSAKYS SLAEAASKAD GLAVIGVLMK VGEANPKLQK VLDALQAIKT KGKRAPFTNF DPSTLLPSSL DFWTYPGS LT HPPLYESVTW IICKESISVS SEQLAQFRSL LSNVEGDNAV PMQHNNRPTQ PLKGRTVRAS F
Purity : >95% by SDS-PAGE
Applications : SDS-PAGE
Storage : Can be stored at 4℃ short term (1-2 weeks). For long term storage, aliquot and store at -20℃ or -70℃. Avoid repeated freezing and thawing cycles.
Concentration : 1 mg/ml (determined by Bradford assay)
Gene Name : CA1 carbonic anhydrase I [ Homo sapiens ]
Official Symbol : CA1
Synonyms : CA1; carbonic anhydrase I; carbonic anhydrase 1; Car1; carbonic anhydrase B; carbonic dehydratase; carbonate dehydratase I; CAB; CA-I;
Gene ID : 759
mRNA Refseq : NM_001128829
Protein Refseq : NP_001122301
MIM : 114800
UniProt ID : P00915
Chromosome Location : 8q21.2
Pathway : C-MYB transcription factor network, organism-specific biosystem;
Function : carbonate dehydratase activity; lyase activity; metal ion binding; zinc ion binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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How does CA1 protein's phosphorylation change during synaptic stimulation? 04/13/2022

CA1 protein's phosphorylation changes with synaptic stimulation, impacting its function.

What impact do post-translational modifications have on CA1 protein's subcellular localization and synaptic function? 08/22/2021

Post-translational modifications can alter CA1 protein's subcellular localization and synaptic function.

How do epigenetic factors influence CA1 protein's function in memory formation? 07/12/2021

Epigenetic factors influence CA1 protein's function in memory formation via modifications.

What is CA1 protein's specific role in regulating memory processes? 07/27/2018

CA1 protein plays a specific role in memory processes, aiding memory formation.

What is CA1 protein's specific role in regulating synaptic plasticity? 01/07/2018

CA1 protein regulates synaptic plasticity by modulating synaptic strength.

How do genetic factors regulate CA1 protein expression? 10/13/2017

Genetic factors regulate CA1 protein expression, affecting its levels.

Can we develop a high-resolution imaging technique to monitor CA1 protein dynamics within dendritic spines? 10/03/2016

Developing high-resolution imaging techniques for monitoring CA1 protein dynamics is feasible.

Customer Reviews (3)

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Reviews
05/19/2022

    Swift protein purification, expedites our research timelines.

    04/23/2021

      Efficient analysis, aids our understanding of protein function.

      09/17/2020

        Reliable protein assays, critical for our scientific investigations.

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