Recombinant Human Carbonic Anhydrase I, His-tagged

• Cat.No.: CA1-2505H
• Product Overview: Recombinant human Carbonic anhydrase1, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography. MW: 31.0 kDa.

Specification

• Description: CA1 is a zinc metalloenzyme that catalyses reversible hydration of CO2 (CO2 + H2O<->HCO3- + H+). This protein is fundamental to many biological processes such as cellular respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, salvia, and gastric acid. It is most abundant in erythrocytes and is a very early marker for erythroid differentiation.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol.
• Molecular Mass: 31.0 kDa (281aa), confirmed by MALDI-TOF
• Protein length: 1-261aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MASPDWGYDD KNGPEQWSKL YPIANGNNQS PVDIKTSETK HDTSLKPISV SYNPATAKEI INVGHSFHVN FEDNDNRSVL KGGPFSDSYR LFQFHFHWGS TNEHGSEHTV DGVKYSAELH VAHWNSAKYS SLAEAASKAD GLAVIGVLMK VGEANPKLQK VLDALQAIKT KGKRAPFTNF DPSTLLPSSL DFWTYPGS LT HPPLYESVTW IICKESISVS SEQLAQFRSL LSNVEGDNAV PMQHNNRPTQ PLKGRTVRAS F
• Purity: >95% by SDS-PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4℃ short term (1-2 weeks). For long term storage, aliquot and store at -20℃ or -70℃. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: CA1 carbonic anhydrase I [ Homo sapiens ]
• Official Symbol: CA1
• Synonyms: CA1; carbonic anhydrase I; carbonic anhydrase 1; Car1; carbonic anhydrase B; carbonic dehydratase; carbonate dehydratase I; CAB; CA-I
• Gene ID: 759
• mRNA Refseq: NM_001128829
• Protein Refseq: NP_001122301
• MIM: 114800
• UniProt ID: P00915
• Chromosome Location: 8q21.2
• Pathway: C-MYB transcription factor network, organism-specific biosystem
• Function: carbonate dehydratase activity; lyase activity; metal ion binding; zinc ion binding

Reviews

05/19/2022

Swift protein purification, expedites our research timelines.

04/23/2021

Efficient analysis, aids our understanding of protein function.

09/17/2020

Reliable protein assays, critical for our scientific investigations.

Q&As

How does CA1 protein's phosphorylation change during synaptic stimulation?

CA1 protein's phosphorylation changes with synaptic stimulation, impacting its function.

What impact do post-translational modifications have on CA1 protein's subcellular localization and synaptic function?

Post-translational modifications can alter CA1 protein's subcellular localization and synaptic function.

How do epigenetic factors influence CA1 protein's function in memory formation?

Epigenetic factors influence CA1 protein's function in memory formation via modifications.

What is CA1 protein's specific role in regulating memory processes?

CA1 protein plays a specific role in memory processes, aiding memory formation.

What is CA1 protein's specific role in regulating synaptic plasticity?

CA1 protein regulates synaptic plasticity by modulating synaptic strength.

How do genetic factors regulate CA1 protein expression?

Genetic factors regulate CA1 protein expression, affecting its levels.

Can we develop a high-resolution imaging technique to monitor CA1 protein dynamics within dendritic spines?

Developing high-resolution imaging techniques for monitoring CA1 protein dynamics is feasible.