Recombinant Human Conserved Helix-Loop-Helix Ubiquitous Kinase, GST-tagged

• Cat.No.: CHUK-1004H
• Product Overview: Full length recombinant human CHUK was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Description: CHUK is a serine/threonine protein kinase that phosphorylates the I-kappa-B protein which is an inhibitor of the transcription factor NF-kappa-B complex. Phosphorylation of I-kappa-B protein triggers the degradation of the inhibitor via the ubiquitination pathway, thereby activating NF-kappa-B complex. CHUK is an essential regulator of NF-kappa-B-dependent gene expression through control of promoter-associated histone phosphorylation after cytokine exposure. CHUK is a critical component of the cytoplasmic transductional-transcriptional processor leading to induction of IFNα production. CHUK is also involved in the epidermis where it antagonizes mitogenic and angiogenic signals and represses tumor progression and metastases.
• Applications: Kinase Assay; Western Blot
• Molecular Weight: 114 kDa
• Expression System: Sf9 insect cells using baculovirus
• Form: Recombinant protein stored in 50 mM Tris-HCl, pH 7.5, 15 0mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM pmsf, 25 % glycerol.
• Specific Activity: 2.5 nmol/min/mg
• Purity: > 75 %
• Concentration: 0.1 ug/ul
• Sequences: Full length
• Storage: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Pathways: AKT phosphorylates targets in the cytosol; Activated TLR4 signalling; Acute myeloid leukemia; Adaptive Immunity Signaling; Adipocytokine signaling pathway; Alternative NF-kappaB pathway; Apoptosis; B Cell Receptor Signaling Pathway; BCR signaling pathway; Canonical NF-kappaB pathway; Chagas disease (American trypanosomiasis); Chemokine signaling pathway; Chronic myeloid leukemia; Class I PI3K signaling events mediated by Akt; Cytokine Signaling in Immune system
• Full Length: Full L.

Gene Information

• Gene Name: CHUK conserved helix-loop-helix ubiquitous kinase [ Homo sapiens ]
• Official Symbol: CHUK
• Synonyms: CHUK; conserved helix-loop-helix ubiquitous kinase; IKK1; IKKA; IKBKA; TCF16; NFKBIKA; IKK-alpha; Inhibitor of nuclear factor kappa-B kinase subunit alpha; inhibitor of nuclear factor kappa-B kinase subunit alpha; TCF-16; IKK-a kinase; I-kappa-B kinase 1; OTTHUMP00000020273; I-kappa-B kinase-alpha; transcription factor 16; IkB kinase alpha subunit; Nuclear factor NFkappaB inhibitor kinase alpha; I-kappa-B kinase alpha; IkappaB kinase; EC 2.7.11.10; Nuclear factor NF-kappa-B inhibitor kinase alpha
• Gene ID: 1147
• mRNA Refseq: NM_001278
• Protein Refseq: NP_001269
• MIM: 600664
• UniProt ID: O15111
• Chromosome Location: 10q24-q25
• Function: ATP binding; IkappaB kinase activity; nucleotide binding; protein binding; protein kinase activity