Recombinant Human EIF2S1 Protein, GST-tagged

• Cat.No.: EIF2S1-3172H
• Product Overview: Human EIF2S1 full-length ORF ( AAH02513.1, 1 a.a. - 315 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM, Feb 2010]
• Source: Wheat Germ
• Species: Human
• Tag: GST
• Molecular Mass: 60.39 kDa
• AA Sequence: MPGLSCRFYQHKFPEVEDVVMVNVR SIAEMGAYVSLLEYNNIEGMILLSE LSRRRIRSINKLIRIGRNECVVVIR VDKEKGYIDLSKRRVSPEEAIKCED KFTKSKTVYSILRHVAEVLEYTKDE QLESLFQRTAWVFDDKYKRPGYGAY DAFKHAVSDPSILDSLDLNEDEREV LINNINRRLTPQAVKIRADIEVACY GYEGIDAVKEALRAGLNCSTENMPI KINLIAPPRYVMTTTTLERTEGLSV LSQAMAVIKEKIEEKRGVFNVQMEP KVVTDTDETELARQMERLERENAEV DGDDDAEEMEAKAED
• Applications: Enzyme-linked Immunoabsorbent Assay
  Western Blot (Recombinant protein)
  Antibody Production
  Protein Array
• Notes: Best use within three months from the date of receipt of this protein.
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: EIF2S1 eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa [ Homo sapiens ]
• Official Symbol: EIF2S1
• Synonyms: EIF2S1; eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa; EIF2, eukaryotic translation initiation factor 2, subunit 1 (alpha, 35kD ); eukaryotic translation initiation factor 2 subunit 1; EIF 2alpha; EIF2A; eIF-2-alpha; eukaryotic translation initiation factor 2 subunit alpha; EIF2; EIF-2; EIF-2A; EIF-2alpha
• Gene ID: 1965
• mRNA Refseq: NM_004094
• Protein Refseq: NP_004085
• MIM: 603907
• UniProt ID: P05198