Recombinant Human EIF2S1 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: EIF2S1-3542H
• Product Overview: EIF2S1 MS Standard C13 and N15-labeled recombinant protein (NP_004085) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2), and the 52-kD EIF2-gamma subunit (EIF2S3). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 36.1 kDa
• AA Sequence: MPGLSCRFYQHKFPEVEDVVMVNVR SIAEMGAYVSLLEYNNIEGMILLSE LSRRRIRSINKLIRIGRNECVVVIR VDKEKGYIDLSKRRVSPEEAIKCED KFTKSKTVYSILRHVAEVLEYTKDE QLESLFQRTAWVFDDKYKRPGYGAY DAFKHAVSDPSILDSLDLNEDEREV LINNINRRLTPQAVKIRADIEVACY GYEGIDAVKEALRAGLNCSTENMPI KINLIAPPRYVMTTTTLERTEGLSV LSQAMAVIKEKIEEKRGVFNVQMEP KVVTDTDETELARQMERLERENAEV DGDDDAEEMEAKAEDTRTRPLEQKL ISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: EIF2S1 eukaryotic translation initiation factor 2 subunit alpha [ Homo sapiens (human) ]
• Official Symbol: EIF2S1
• Synonyms: EIF2S1; eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa; EIF2, eukaryotic translation initiation factor 2, subunit 1 (alpha, 35kD ); eukaryotic translation initiation factor 2 subunit 1; EIF 2alpha; EIF2A; eIF-2-alpha; eukaryotic translation initiation factor 2 subunit alpha; EIF2; EIF-2; EIF-2A; EIF-2alpha
• Gene ID: 1965
• mRNA Refseq: NM_004094
• Protein Refseq: NP_004085
• MIM: 603907
• UniProt ID: P05198