Recombinant Human GPT Protein, Myc/DDK-tagged, C13 and N15-labeled
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| Cat.No. : | GPT-4845H |
| Product Overview : | GPT MS Standard C13 and N15-labeled recombinant protein (NP_005300) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells. |
| Description : | This gene encodes cytosolic alanine aminotransaminase 1 (ALT1); also known as glutamate-pyruvate transaminase 1. This enzyme catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase. |
| Source : | HEK293 |
| Species : | Human |
| Tag : | Myc/DDK |
| Molecular Mass : | 54.6 kDa |
| AA Sequence : | MASSTGDRSQ AVRHGLRAKV LTLDGMNPRV RRVEYAVRGP IVQRALELEQ ELRQGVKKPF TEVIRANIGD AQAMGQRPIT FLRQVLALCV NPDLLSSPNF PDDAKKRAER ILQACGGHSL GAYSVSSGIQ LIREDVARYI ERRDGGIPAD PNNVFLSTGA SDAIVTVLKL LVAGEGHTRT GVLIPIPQYP LYSATLAELG AVQVDYYLDE ERAWALDVAE LHRALGQARD HCRPRALCVI NPGNPTGQVQ TRECIEAVIR FAFEERLFLL ADEVYQDNVY AAGSQFHSFK KVLMEMGPPY AGQQELASFH STSKGYMGEC GFRGGYVEVV NMDAAVQQQM LKLMSVRLCP PVPGQALLDL VVSPPAPTDP SFAQFQAEKQ AVLAELAAKA KLTEQVFNEA PGISCNPVQG AMYSFPRVQL PPRAVERAQE LGLAPDMFFC LRLLEETGIC VVPGSGFGQR EGTYHFRMTI LPPLEKLRLL LEKLSRFHAK FTLEYSTRTR PLEQKLISEE DLAANDILDY KDDDDKV |
| Purity : | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : | Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : | Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : | 50 μg/mL as determined by BCA |
| Storage Buffer : | 100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
| SDS-PAGE : |
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| Gene Name : | GPT glutamic--pyruvic transaminase [ Homo sapiens (human) ] |
| Official Symbol : | GPT |
| Synonyms : | GPT; glutamic-pyruvate transaminase (alanine aminotransferase); alanine aminotransferase 1; ALT1; GPT1; GPT 1; glutamic-alanine transaminase 1; glutamic--alanine transaminase 1; glutamic--pyruvic transaminase 1; glutamate pyruvate transaminase 1; AAT1; |
| Gene ID : | 2875 |
| mRNA Refseq : | NM_005309 |
| Protein Refseq : | NP_005300 |
| MIM : | 138200 |
| UniProt ID : | P24298 |
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◆ Native Protein
| GPT-189P | Native Porcine GPT protein | +Inquiry |
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◆ Lysates
| GPT-721RCL | Recombinant Rat GPT cell lysate | +Inquiry |
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