Recombinant Human GPT Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: GPT-4845H
• Product Overview: GPT MS Standard C13 and N15-labeled recombinant protein (NP_005300) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: This gene encodes cytosolic alanine aminotransaminase 1 (ALT1); also known as glutamate-pyruvate transaminase 1. This enzyme catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 54.6 kDa
• AA Sequence: MASSTGDRSQAVRHGLRAKVLTLDG MNPRVRRVEYAVRGPIVQRALELEQ ELRQGVKKPFTEVIRANIGDAQAMG QRPITFLRQVLALCVNPDLLSSPNF PDDAKKRAERILQACGGHSLGAYSV SSGIQLIREDVARYIERRDGGIPAD PNNVFLSTGASDAIVTVLKLLVAGE GHTRTGVLIPIPQYPLYSATLAELG AVQVDYYLDEERAWALDVAELHRAL GQARDHCRPRALCVINPGNPTGQVQ TRECIEAVIRFAFEERLFLLADEVY QDNVYAAGSQFHSFKKVLMEMGPPY AGQQELASFHSTSKGYMGECGFRGG YVEVVNMDAAVQQQMLKLMSVRLCP PVPGQALLDLVVSPPAPTDPSFAQF QAEKQAVLAELAAKAKLTEQVFNEA PGISCNPVQGAMYSFPRVQLPPRAV ERAQELGLAPDMFFCLRLLEETGIC VVPGSGFGQREGTYHFRMTILPPLE KLRLLLEKLSRFHAKFTLEYSTRTR PLEQKLISEEDLAANDILDYKDDDD KV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: GPT glutamic--pyruvic transaminase [ Homo sapiens (human) ]
• Official Symbol: GPT
• Synonyms: GPT; glutamic-pyruvate transaminase (alanine aminotransferase); alanine aminotransferase 1; ALT1; GPT1; GPT 1; glutamic-alanine transaminase 1; glutamic--alanine transaminase 1; glutamic--pyruvic transaminase 1; glutamate pyruvate transaminase 1; AAT1
• Gene ID: 2875
• mRNA Refseq: NM_005309
• Protein Refseq: NP_005300
• MIM: 138200
• UniProt ID: P24298