Recombinant Full Length Human GPT Protein, C-Flag-tagged

• Cat.No.: GPT-1066HFL
• Product Overview: Recombinant Full Length Human GPT Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes cytosolic alanine aminotransaminase 1 (ALT1); also known as glutamate-pyruvate transaminase 1. This enzyme catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 54.5 kDa
• AA Sequence: MASSTGDRSQAVRHGLRAKVLTLDG MNPRVRRVEYAVRGPIVQRALELEQ ELRQGVKKPFTEVIRANIGD AQAMGQRPITFLRQVLALCVNPDLL SSPNFPDDAKKRAERILQACGGHSL GAYSVSSGIQLIREDVARYI ERRDGGIPADPNNVFLSTGASDAIV TVLKLLVAGEGHTRTGVLIPIPQYP LYSATLAELGAVQVDYYLDE ERAWALDVAELHRALGQARDHCRPR ALCVINPGNPTGQVQTRECIEAVIR FAFEERLFLLADEVYQDNVY AAGSQFHSFKKVLMEMGPPYAGQQE LASFHSTSKGYMGECGFRGGYVEVV NMDAAVQQQMLKLMSVRLCP PVPGQALLDLVVSPPAPTDPSFAQF QAEKQAVLAELAAKAKLTEQVFNEA PGISCNPVQGAMYSFPRVQL PPRAVERAQELGLAPDMFFCLRLLE ETGICVVPGSGFGQREGTYHFRMTI LPPLEKLRLLLEKLSRFHAK
  FTLEYSTRTRPLEQKLISEEDLAAN DILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome
• Protein Pathways: Alanine, aspartate and glutamate metabolism, Metabolic pathways

Gene Information

• Gene Name: GPT glutamic--pyruvic transaminase [ Homo sapiens (human) ]
• Official Symbol: GPT
• Synonyms: ALT; AAT1; ALT1; GPT1; SGPT
• Gene ID: 2875
• mRNA Refseq: NM_005309.3
• Protein Refseq: NP_005300.1
• MIM: 138200
• UniProt ID: P24298