Active Recombinant Human IDH2 protein, His-tagged

• Cat.No.: IDH2-119H
• Product Overview: Recombinant Human IDH2 protein(40-452 aa), fused with N-terminal His tag, was expressed in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 40-452 aa
• Form: PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
• Bio-activity: Isocitrate Dehydrogenase 2 (IDH2) catalyzes the oxidative decarboxylation of isocitrate to alpha-ketoglutarate. There are two subclasses in the IDH family, one of them utilizing NADP+ as the electron acceptor and the other using NAD+. The protein encoded by this gene is the NADP+ dependent isocitrate dehydrogenase found in the mitochondrial. The activity of recombinant human IDH2 was measured by the ability to oxidatively decarboxylate isocitrate to 2-oxoglutarate. The reaction was performed in 25 mM Tris, 0.5 mM MnCl2, 5 mM DTT, pH 7.5 (Assay Buffer), initiated by addition 50 μ L of various concentrations of IDH2 (diluted by Assay Buffer) to 50 µL of substrate mixture consisted of 1 mM NADP+ and 2 mM isocitric acid. The final well serves as a negative control with no IDH2, replaced with 50 μ l assay buffer. Incubated at 25°C for 5min, then read at a wavelength of 340 nm. The specific activity of recombinant human IDH2 is >200 Isocitrate Dehydrogenase 2 (IDH2) catalyzes the oxidative decarboxylation of isocitrate to alpha-ketoglutarate. There are two subclasses in the IDH family, one of them utilizing NADP+ as the electron acceptor and the other using NAD+. The protein encoded by this gene is the NADP+ dependent isocitrate dehydrogenase found in the mitochondrial. The activity of recombinant human IDH2 was measured by the ability to oxidatively decarboxylate isocitrate to 2-oxoglutarate. The reaction was performed in 25 mM Tris, 0.5 mM MnCl2, 5 mM DTT, pH 7.5 (Assay Buffer), initiated by addition 50 μ L of various concentrations of IDH2 (diluted by Assay Buffer) to 50 µL of substrate mixture consisted of 1 mM NADP+ and 2 mM isocitric acid. The final well serves as a negative control with no IDH2, replaced with 50 μ l assay buffer. Incubated at 25°C for 5min, then read at a wavelength of 340 nm. The specific activity of recombinant human IDH2 is >200 pmol/min/µg.
• Molecular Mass: 50.3kDa
• Endotoxin: <1.0EU per 1μg (determined by the LAL method).
• Purity: >90%
• Storage: Avoid repeated freeze/thaw cycles. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 month.
• Reconstitution: Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: IDH2 isocitrate dehydrogenase 2 (NADP+), mitochondrial [ Homo sapiens ]
• Official Symbol: IDH2
• Synonyms: IDH2; isocitrate dehydrogenase 2 (NADP+), mitochondrial; isocitrate dehydrogenase [NADP], mitochondrial; NADP(+)-specific ICDH; oxalosuccinate decarboxylase; IDH; IDP; IDHM; IDPM; ICD-M; D2HGA2; mNADP-IDH
• Gene ID: 3418
• mRNA Refseq: NM_002168
• Protein Refseq: NP_002159
• MIM: 147650
• UniProt ID: P48735