Recombinant Full Length Human IDH2 Protein, C-Flag-tagged

• Cat.No.: IDH2-1044HFL
• Product Overview: Recombinant Full Length Human IDH2 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex. Alternative splicing results in multiple transcript variants.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 46.6 kDa
• AA Sequence: MAGYLRVVRSLCRASGSRPAWAPAA LTAPTSQEQPRRHYADKRIKVAKPV VEMDGDEMTRIIWQFIKEKL ILPHVDIQLKYFDLGLPNRDQTDDQ VTIDSALATQKYSVAVKCATITPDE ARVEEFKLKKMWKSPNGTIR NILGGTVFREPIICKNIPRLVPGWT KPITIGRHAHGDQYKATDFVADRAG TFKMVFTPKDGSGVKEWEVY NFPAGGVGMGMYNTDESISGFAHSC FQYAIQKKWPLYMSTKNTILKAYDG RFKDIFQEIFDKHYKTDFDK NKIWYEHRLIDDMVAQVLKSSGGFV WACKNYDGDVQSDILAQGFGSLGLM TSVLVCPDGKTIEAEAAHGT VTRHYREHQKGRPTSTNPIASIFAW TRGLEHRGKLDGNQDLIRFAQMLEK VCVETVESGAMTKDLAGCIH
  GLSNVKLNEHFLNTTDFLDTIKSNL DRALGRQTRTRPLEQKLISEEDLAA NDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Pathways: Citrate cycle (TCA cycle), Glutathione metabolism, Metabolic pathways

Gene Information

• Gene Name: IDH2 isocitrate dehydrogenase (NADP(+)) 2 [ Homo sapiens (human) ]
• Official Symbol: IDH2
• Synonyms: IDH; IDP; IDHM; IDPM; ICD-M; IDH-2; D2HGA2; mNADP-IDH
• Gene ID: 3418
• mRNA Refseq: NM_002168.4
• Protein Refseq: NP_002159.2
• MIM: 147650
• UniProt ID: P48735