Recombinant Full Length Human IDH2(R140K) Protein, Flag tagged

• Cat.No.: IDH2-115H
• Product Overview: Recombinant IDH2 (R140Q) protein was expressed in baculovirus system as the full length protein (accession number NP_002159.2) with a point mutation Arg140Gln and a C-terminal FLAG-Tag.

Specification

• Species: Human
• Source: Insect Cells
• Tag: Flag
• Protein Length: 1-452 aa
• Description: IDH2 (Isocitrate Dehydrogenase (NADP(+)) 2, Mitochondrial, also known as MNADP-IDH, D2HGA2, IDHM, IDH, IDPM, IDC-M) is a member of isocitrate dehydrogenases, which catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. IDH2 is found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex. Mutations in IDH1 and IDH2 frequently occur in grade II-III gliomas, secondary glioblastomas and acute myeloid leukemias (AML). To date, mutations in at least four active site arginine residues IDH1 R100, IDH1 R132, IDH2 R140, and IDH2 R172 have been shown to result in the neomorphic production of R(-)-2-hydroxyglutarate (2HG), although these mutants lack the wild-type enzyme's ability to convert isocitrate to α-ketoglutarate (α-KG, 2OG).
• Molecular Mass: 52.6 kDa
• Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 centigrade to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
• Storage Buffer: 25 mM HEPES-NaOH pH 7.5, 300 mM NaCI, 10% glycerol,0.04% Triton X-100, and 0.5 mM TCEP.
• Concentration: 0.35 μg/μL

Gene Information

• Gene Name: IDH2 isocitrate dehydrogenase 2 (NADP+), mitochondrial [ Homo sapiens (human) ]
• Official Symbol: IDH2
• Synonyms: IDH2; isocitrate dehydrogenase 2 (NADP+), mitochondrial; isocitrate dehydrogenase [NADP], mitochondrial; NADP(+)-specific ICDH; oxalosuccinate decarboxylase; IDH; IDP; IDHM; IDPM; ICD-M; D2HGA2; mNADP-IDH
• Gene ID: 3418
• mRNA Refseq: NM_002168
• Protein Refseq: NP_002159
• MIM: 147650
• UniProt ID: P48735