Recombinant Human IL13, His-Biotin-tagged

Cat.No. : IL13-16H
Product Overview : A DNA sequence encoding the mature form of human IL-13 (Leu25 - Asn146) with 6 His tag on the N-Terminus was expressed in E. Coli. This protein was purified by Ni-NTA column. That protein was conjugated with water soluble biotin.
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Description : This gene encodes an immunoregulatory cytokine produced primarily by activated Th2 cells. This cytokine is involved in several stages of B-cell maturation and differentiation. It up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. This cytokine down-regulates macrophage activity, thereby inhibits the production of pro-inflammatory cytokines and chemokines. This cytokine is found to be critical to the pathogenesis of allergen-induced asthma but operates through mechanisms independent of IgE and eosinophils. This gene, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL4.
Source : E. Coli
Species : Human
Tag : His-Biotin
Form : Lyophilized 50 ug of human IL-13 Biotinylated in 50 ul of PBS Carry free.
Molecular Mass : 14 KD
AA Sequence : Human IL-13 (Leu25 - Asn146) Biotinylated
Purity : >95%, by SDS-PAGE
Applications : ELISA
Storage : Store lyophilized protein at -20 °C or -70 °C. Protect it from light. Lyophilized protein is stable for up to 6 months from date of receipt at - 20 °C to -70 °C. Upon reconstitution, this protein can be stored at -20 °C for a few weeks or at -70 °C in a manual defrost freezer for long term storage (six months). Aliquot reconstituted protein to avoid repeated freezing / thawing cycles.
Reconstitution : Add 100 ul deionized water to the vial to prepare a working stock solution at 500 ug/mL. Allow to set at least 30 minutes at 4 °C, mix well.
Gene Name : IL13 interleukin 13 [ Homo sapiens (human) ]
Official Symbol : IL13
Synonyms : IL13; P600; IL-13; interleukin 13; interleukin-13
Gene ID : 3596
mRNA Refseq : NM_002188
Protein Refseq : NP_002179
MIM : 147683
UniProt ID : P35225
Chromosome Location : 5q31
Pathway : Cytokine-cytokine receptor interaction; Cytokines and Inflammatory Response; Fc epsilon RI signaling pathway
Function : cytokine activity; interleukin-13 receptor binding; protein binding

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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How does flow cytometry help quantify IL13 receptor expression on different cell populations? 12/29/2022

Flow cytometry quantifies IL13 receptor expression on cells.

What are the benefits of using transgenic animal models to study IL13's in vivo functions? 07/05/2022

Transgenic models illuminate IL13's in vivo functions.

Could you explain the role of ELISA in quantifying IL13 secretion levels in cell cultures? 02/23/2022

ELISA quantifies IL13 secretion in cell cultures accurately.

How can siRNA knockdown experiments reveal IL13's effects on specific cellular processes? 06/13/2021

siRNA knockdown uncovers IL13's specific cellular effects.

How does co-immunoprecipitation identify interacting partners in IL13-mediated signaling complexes? 07/28/2019

Co-immunoprecipitation identifies IL13 signaling partners.

What techniques can differentiate IL13-mediated signaling pathways from IL4 pathways? 07/23/2019

Differential techniques distinguish IL13 and IL4 signaling pathways.

Could you elaborate on the use of reporter gene assays to assess IL13-driven transcriptional activity? 12/16/2018

Reporter gene assays gauge IL13-driven transcription.

Can you describe the applications of microarray analysis in profiling IL13-induced gene expression changes? 11/14/2018

Microarray analysis profiles IL13-induced gene expression.

What experimental methods determine the impact of IL13 on tissue remodeling processes? 08/04/2018

Experimental methods reveal IL13's impact on tissue remodeling.

How does site-directed mutagenesis help identify critical residues in IL13 receptor binding? 07/04/2018

Site-directed mutagenesis identifies key residues in IL13 receptor binding.

Customer Reviews (3)

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Reviews
11/29/2021

    Effective in Th2 cell differentiation.

    04/22/2020

      Good for cytokine assays.

      01/26/2019

        High yield in expression.

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