Active Recombinant Human IL4R Protein, His & Avi-tagged, Biotinylated

• Cat.No.: IL4R-588H
• Product Overview: Biotinylated Recombinant Human IL4R Protein (Met 26 - His 232) (NP_000409.1), fused with an Avi tag at the C-terminus, followed by a polyhistidine tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: His & Avi
• Predicted N Terminal: Met 26
• Form: Lyophilized from sterile PBS, pH7.4, 10% trehalose.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized Human IL-4, Tag Free at 5μg/mL (100 μL/well) can bind Biotinylated Human IL-4 R alpha, His Tag with a linear range of 0.005-0.078 μg/mL.
• Molecular Mass: Recombinant Human IL4R Protein has a calculated MW of 26.9 kDa. The protein migrates as 40-53 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.
• Protein length: Met 26 - His 232
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: IL4R interleukin 4 receptor [ Homo sapiens ]
• Official Symbol: IL4R
• Synonyms: IL4R; interleukin 4 receptor; interleukin-4 receptor subunit alpha; CD124; IL-4 receptor subunit alpha; interleukin-4 receptor alpha chain; IL4RA; IL-4RA
• Gene ID: 3566
• mRNA Refseq: NM_000418
• Protein Refseq: NP_000409
• MIM: 147781
• UniProt ID: P24394
• Chromosome Location: 16p12.1-p11.2
• Pathway: Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Hematopoietic cell lineage, organism-specific biosystem; Hematopoietic cell lineage, conserved biosystem; IL-4 signaling Pathway, organism-specific biosystem; IL4-mediated signaling events, organism-specific biosystem; Inflammatory Response Pathway, organism-specific biosystem
• Function: insulin receptor substrate binding; interleukin-4 receptor activity; protein binding; receptor activity; receptor signaling protein activity