Recombinant Human IL4R cell lysate

• Cat.No.: IL4R-2719HCL
• Product Overview: Human IL4R / CD124 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls

Specification

• Source: Human cells
• Species: Human
• Preparation method: Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
• Lysis buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Quality control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue
• Recommended Usage: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
• Stability: Samples are stable for up to twelve months from date of receipt at -80°C
• Storage Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Storage Instruction: Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
• Tag: Non

Gene Information

• Gene Name: IL4R interleukin 4 receptor [ Homo sapiens ]
• Official Symbol: IL4R
• Synonyms: IL4R; interleukin 4 receptor; interleukin-4 receptor subunit alpha; CD124; IL-4 receptor subunit alpha; interleukin-4 receptor alpha chain; IL4RA; IL-4RA
• Gene ID: 3566
• mRNA Refseq: NM_000418
• Protein Refseq: NP_000409
• MIM: 147781
• UniProt ID: P24394
• Chromosome Location: 16p12.1-p11.2
• Pathway: Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Hematopoietic cell lineage, organism-specific biosystem; Hematopoietic cell lineage, conserved biosystem; IL-4 signaling Pathway, organism-specific biosystem; IL4-mediated signaling events, organism-specific biosystem; Inflammatory Response Pathway, organism-specific biosystem
• Function: insulin receptor substrate binding; interleukin-4 receptor activity; protein binding; receptor activity; receptor signaling protein activity

Reviews

06/30/2022

Easy to attach to the surface of cells or materials.

09/29/2021

It has growth factor activity to promote cell growth and proliferation.

02/27/2019

In animals or humans caused by the prone production of antibodies.

Q&As

How about function of IL4R?

IL4R is a synthetic protein that mimics the IL-4 signaling pathway in nature and binds to the IL-4 receptor in cells, which can be used to study the IL-4 signaling pathway and related diseases.

What is the production technology of IL4R?

Common IL4R production technologies include prokaryotic expression systems and mammalian cell expression systems.

Which application of IL4R in tumor therapy?

This protein is used in tumor immunotherapy, which can promote anti-tumor immune response and enhance the anti-tumor effect of cytotoxic T lymphocytes.

In what fields does IL4R have applications?

It is mainly used in immunology, drug research and other fields. It is also widely used in vaccines, biotechnology, cancer treatment and so on.

Are there have application of IL4R in the treatment of asthma?

IL4R is used to treat severe pheochromocytome-induced aerodoritis and was approved by the FDA as the first drug to treat this disease.

Can IL4R be administered orally?

IL4R cannot generally be administered orally, and needs to be administered by injection or intravenous drip.