Active Recombinant Human IL4R Protein, His & Avi-tagged, Biotinylated, Low Endotoxin

• Cat.No.: IL4R-588H
• Product Overview: Biotinylated Active Recombinant Human IL4R Protein(P24394-1)(Met26-His232) is expressed from HEK293 with His tag and Avi tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: 26-232 aa
• Conjugation/Label: Biotin
• Form: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4).
• Bio-activity: Immobilized Human IL-4, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-4 R alpha, His Avi Tag with the EC50 of 50.5ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 26.64 kDa. Due to glycosylation, the protein migrates to 58-68 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: IL4R interleukin 4 receptor [ Homo sapiens ]
• Official Symbol: IL4R
• Synonyms: IL4R; interleukin 4 receptor; interleukin-4 receptor subunit alpha; CD124; IL-4 receptor subunit alpha; interleukin-4 receptor alpha chain; IL4RA; IL-4RA
• Gene ID: 3566
• mRNA Refseq: NM_000418
• Protein Refseq: NP_000409
• MIM: 147781
• UniProt ID: P24394