Recombinant Human PDE4A, GST-tagged, Active

Cat.No. : PDE4A-475H
Product Overview : Recombinanthuman PDE4A (332-end) was expressed bybaculovirusinSf9 insect cellsusing an N-terminal GST tag. MW=110kDa.
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Cat. No. : PDE4A-475H
Description : PDE4A is a member of the phosphodiesterase family of proteins that play a critical role in regulating intracellular levels of cAMP. In vitro phosphorylation of PDE4A by the PKA-catalytic subunit increases the enzyme"s sensitivity to Mg2+, leading to a 4-fold increase in cAMP hydrolysis without affecting the Km. PDE4 is widely expressed in brain tumors and promotes their growth and treatment with PDE4A inhibitor Rolipram overcomes tumor resistance and mediates tumor regression. PDE4A expression is altered in patients with bipolar disorder and is a new therapeutic avenue for treatment of this disorder.
Source : Sf9 insect cells using baculovirus.
Sequence : 332-end.
Applications : PDE Assay.
Storage And Stability : Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
22q13.33 : 19p13.2
Gene Name : PDE4A phosphodiesterase 4A, cAMP-specific (phosphodiesterase E2 dunce homolog, Drosophila) [ Homo sapiens ]
Synonyms : PDE4A; phosphodiesterase 4A, calmodulin-dependent; cAMP-specific phosphodiesterase; phosphodiesterase 4A; phosphodiesterase 4A, cAMP-specific (dunce; phosphodiesterase E2 dunce homolog, Drosophila; phosphodiesterase isozyme 4; PDE4; DPDE2; PDE46; EC 3.1.4.17
Gene ID : 5141
mRNA Refseq : NM_001111307
Protein Refseq : NP_001104777
MIM : 600126
UniProt ID : P27815
Pathway : Progesterone-mediated oocyte maturation; Purine metabolism; Signaling by GPCR; Insulin signaling pathway
Function : 3",5"-cyclic-AMP phosphodiesterase activity; cGMP-inhibited cyclic-nucleotide phosphodiesterase activity; hydrolase activity

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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12/05/2022

    PDE4A requires less storage environment.

    07/26/2022

      The expression of PDE4A was stable.

      02/08/2022

        It has the advantage of easy operation in the expression system and can improve the expression efficiency.

        Q&As (6)

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        How effective are PDE4A inhibitors in the treatment of asthma? 04/12/2022

        The inhibitors have been used in the treatment of asthma, and studies have shown that they can significantly reduce the number of asthma attacks and the use of drug D.

        How to introduce the structure of PDE4A? 03/30/2022

        This protein has multiple conserved regions and different subtypes, which is of great significance for the selective inhibition of drugs.

        What is a selective PDE4A inhibitor? 12/25/2021

        Selective PDE4A inhibitors refer to drug molecules that target only the PDE4A protein and do not inhibit other PDE4 homologs.

        What is the mechanism by which PDE4A regulates T cell-mediated autoimmune responses? 05/30/2021

        It plays an important role in the activation and regulation of T cells by regulating cAMP levels, and is involved in T cell-mediated autoimmune responses.

        Are levels of PDE4A associated with sleep disturbances? 02/12/2021

        Studies have shown that PDE4A depletion may be related to sleep-wake behavior and time stress response, but its specific role needs to be further studied.

        What is the expression pattern of PDE4A in T cells? 08/27/2020

        In T cells, PDE4A is mainly expressed in the cytoplasm and peripheral regions of the nuclear membrane.

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