Recombinant Human PDE4A 293 Cell Lysate

• Cat.No.: PDE4A-3352HCL

Specification

• Description: Antigen standard for phosphodiesterase 4A, cAMP-specific (phosphodiesterase E2 dunce homolog, Drosophila) (PDE4A), transcript variant 4 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
• Tag: Non

Gene Information

• Gene Name: PDE4A phosphodiesterase 4A, cAMP-specific [ Homo sapiens ]
• Official Symbol: PDE4A
• Synonyms: PDE4A; phosphodiesterase 4A, cAMP-specific; DPDE2, phosphodiesterase 4A, cAMP specific (dunce (Drosophila) homolog phosphodiesterase E2); cAMP-specific 3,5-cyclic phosphodiesterase 4A; phosphodiesterase E2 dunce homolog (Drosophila); phosphodiesterase isozyme 4; phosphodiesterase 4A, cAMP-specific (dunce; phosphodiesterase E2 dunce homolog, Drosophila; PDE4; DPDE2; PDE46
• Gene ID: 5141
• mRNA Refseq: NM_001111307
• Protein Refseq: NP_001104777
• MIM: 600126
• UniProt ID: P27815
• Chromosome Location: 19p13.2
• Pathway: DARPP-32 events, organism-specific biosystem; G Protein Signaling Pathways, organism-specific biosystem; G alpha (s) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; Morphine addiction, organism-specific biosystem; Morphine addiction, conserved biosystem; Opioid Signalling, organism-specific biosystem
• Function: 3,5-cyclic-AMP phosphodiesterase activity; hydrolase activity; metal ion binding; phosphoric diester hydrolase activity; protein binding

Reviews

12/05/2022

PDE4A requires less storage environment.

07/26/2022

The expression of PDE4A was stable.

02/08/2022

It has the advantage of easy operation in the expression system and can improve the expression efficiency.

Q&As

How effective are PDE4A inhibitors in the treatment of asthma?

The inhibitors have been used in the treatment of asthma, and studies have shown that they can significantly reduce the number of asthma attacks and the use of drug D.

How to introduce the structure of PDE4A?

This protein has multiple conserved regions and different subtypes, which is of great significance for the selective inhibition of drugs.

What is a selective PDE4A inhibitor?

Selective PDE4A inhibitors refer to drug molecules that target only the PDE4A protein and do not inhibit other PDE4 homologs.

What is the mechanism by which PDE4A regulates T cell-mediated autoimmune responses?

It plays an important role in the activation and regulation of T cells by regulating cAMP levels, and is involved in T cell-mediated autoimmune responses.

Are levels of PDE4A associated with sleep disturbances?

Studies have shown that PDE4A depletion may be related to sleep-wake behavior and time stress response, but its specific role needs to be further studied.

What is the expression pattern of PDE4A in T cells?

In T cells, PDE4A is mainly expressed in the cytoplasm and peripheral regions of the nuclear membrane.