Recombinant Human PTK-2 Protein Tyrosine Kinase 2, His-tagged

• Cat.No.: PTK2-1901H
• Product Overview: Recombinant Human PTK2 (a.a. 411-686) with N-terminal His tag, MW 36kDa, expressed in an Baculovirus infected Sf9 cell expression system.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: His
• Protein Length: 411-686 a.a.
• Description: PTK2 is a cytoplasmic proteintyrosine kinase which is found concentrated in the focal adhesions.Activation of this gene may be an important early step in cell growth andintracellular signal transduction pathways triggered in response to certainneural peptides or to cell interactions with the extracellular matrix.
• Form: 25 mM Tris-HCl, pH8.0 + 100 mM NaCl + 0.05% Tween-20 + 50% glycerol + 3 mM DTT.
• Purity: > 95% by SDS-PAGE
• Specific Activity: 100 U/mg. One unitis defined as the amount of enzyme that will phosphorylate 1 nmol of tyrosinesubstrate per minute at pH 7.4 and 30°C. Assay buffer: 50 mM HEPES, pH 7.4 +3 mM MgCl2 + 3 mM MnCl2 + 1 mM DTT + 3 ugNa-orthovanadate + 0.1 mM ATP + 30 ug/ml Poly (Glu:Tyr)4:1 substrate + 1 ug/ml recombinant PTK2.
• Storage: > 6 months at -80°C.
• OfficialSymbol: PTK2

Gene Information

• Gene Name: PTK2 PTK2 protein tyrosinekinase 2 [ Homo sapiens ]
• Synonyms: PTK2; PTK-2 protein tyrosinekinase 2; FAK; FADK; FAK1; pp125FAK; Focal adhesion kinase 1; FADK 1;Protein-tyrosine kinase 2; Protein-tyrosine kinase 2; EC 2.7.10.2
• Gene ID: 5747
• mRNA Refseq: NM_005607
• Protein Refseq: NP_005598
• MIM: 600758
• UniProt ID: Q05397
• Chromosome Location: 8q24
• Pathway: Axon guidance; ErbB signalingpathway; Chemokine signaling pathway; Focal adhesion; Leukocytetransendothelial migration; Pathways in cancer; Regulation of actincytoskeleton; Small cell lung cancer; VEGF signaling pathway; Apoptosis; Axonguidance; Hemostasis; Integrin cell surface interactions
• Function: ATP binding; SH2 domainbinding; non-membrane spanning protein tyrosine kinase activity; nucleotidebinding; protein binding; signal transducer activity; transferase activity

Citation

Glycolipid iGb3 feedback amplifies innate immune responses via CD1d reverse signaling

Journal: Cell Research    PubMed ID: 30514903    Data: 2019/1/1

Authors: Xingguang Liu, Peng Zhang, Xuetao Cao

Article Snippet:GST-tagged IKKβ or TBK1 protein (Abcam) was incubated with recombinant Pyk2 protein (Creative BioMart) at 4 °C for 30 min, further incubated with glutathione-Sepharose 4B (Pierce) for 2 h in immunoprecipitation buffer.. After washing 3 times, the precipitates were subjected to immunoblot analysis.After washing 3 times, the precipitates were subjected to immunoblot analysis.