Recombinant Human/Mouse/Rat/Porcine NTF3 Protein
Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.
|Cat.No. :||NTF3-30H Optional Service: Optional requirements on this protein|
|Product Overview :||Recombinant human/mouse/rat/porcine NT-3 protein without tag was expressed in E. coli.
Animal-free (AOF) and carrier protein-free.
Mass spectrometry: single species with expected mass.
Recovery from stock vial: >95%
|Description :||The protein encoded by this gene is a member of the neurotrophin family, that controls survival and differentiation of mammalian neurons. This protein is closely related to both nerve growth factor and brain-derived neurotrophic factor. It may be involved in the maintenance of the adult nervous system, and may affect development of neurons in the embryo when it is expressed in human placenta. NTF3-deficient mice generated by gene targeting display severe movement defects of the limbs. The mature peptide of this protein is identical in all mammals examined including human, pig, rat and mouse.|
|Source :||E. coli|
|Molecular Mass :||27.3 kDa dimer, 13.7 kDa monomer|
|Endotoxin :||<0.005 EU/μg protein (below level of detection)|
|Applications :||Induced pluripotent and embryonic Stem cell Differentiation and maintenance|
|Storage :||Resuspend in 10mM HCl (Reconstitution solution A) at >100 μg/mL, prepare single-use aliquots, add carrier protein if desired and store frozen at -20 centigrade (short-term) or -80 centigrade (long-term)|
|Storage Buffer :||Lyophilized from acetonitrile/TFA|
The bioactivity of NT-3 is measured using a luciferase reporter assay in HEK293T cells co-transfected with the TrkA receptor.
Human NT-3migrates mainly as a single band at 13.7 kDa in non-reducingconditions and upon reduction (R). Purified recombinant protein was resolved using 15% w/v SDS-PAGE in reducedand non-reducedconditions and stained with Coomassie Brilliant Blue R-250. A faint band visible at 27.3 kDa in NR and R conditions, corresponds to the non-covalently linked NT-3 dimer. No contaminating bands are visible.
|Gene Name :||NTF3 neurotrophin 3 [ Homo sapiens (human) ]|
|Official Symbol :||NTF3|
|Synonyms :||NTF3; neurotrophin 3; NT3; HDNF; NGF2; NT-3; NGF-2; neurotrophin-3; nerve growth factor 2; neurotrophic factor|
|Gene ID :||4908|
|mRNA Refseq :||NM_002527|
|Protein Refseq :||NP_002518|
|UniProt ID :||P20783|
◆ Recombinant Protein
|Ntf3-1867M||Recombinant Mouse Ntf3 Protein, His-tagged||+Inquiry|
|NTF3-088H||Recombinant Human NTF3 Protein||+Inquiry|
|Ntf3-1868M||Recombinant Mouse Ntf3 Protein, His-tagged||+Inquiry|
|NT3-01H||Recombinant Human NTF3 Protein||+Inquiry|
|NTF3-216H||Recombinant Human/Mouse NTF3 Protein||+Inquiry|
◆ Native Protein
|NTF3-29249TH||Native Human NTF3||+Inquiry|
|NTF3-3671HCL||Recombinant Human NTF3 293 Cell Lysate||+Inquiry|
|NTF3-3670HCL||Recombinant Human NTF3 293 Cell Lysate||+Inquiry|