Recombinant Human NTF3 293 Cell Lysate

• Cat.No.: NTF3-3670HCL

Specification

• Description: Antigen standard for neurotrophin 3 (NTF3), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
• Tag: Non

Gene Information

• Gene Name: NTF3 neurotrophin 3 [ Homo sapiens ]
• Official Symbol: NTF3
• Synonyms: NTF3; neurotrophin 3; neurotrophin-3; NGF2; NT-3; neurotrophic factor; nerve growth factor 2; NT3; HDNF; NGF-2; MGC129711
• Gene ID: 4908
• mRNA Refseq: NM_002527
• Protein Refseq: NP_002518
• MIM: 162660
• UniProt ID: P20783
• Chromosome Location: 12p13
• Pathway: MAPK signaling pathway, organism-specific biosystem; MAPK signaling pathway, conserved biosystem; Neurotrophic factor-mediated Trk receptor signaling, organism-specific biosystem; Neurotrophin signaling pathway, organism-specific biosystem; Neurotrophin signaling pathway, conserved biosystem; Trk receptor signaling mediated by the MAPK pathway, organism-specific biosystem; p75(NTR)-mediated signaling, organism-specific biosystem
• Function: growth factor activity; nerve growth factor binding; neurotrophin receptor binding; receptor binding