Active Recombinant Human GPC3 Protein(495S/A), hFc-tagged

Cat.No. : GPC3-138H
Product Overview : Recombinant Human GPC3 Protein(NP_004475.1, with mutation Ser 495 Ala) (Met1-Ser550) was expressed in HEK293, fused with the Fc region of human IgG1 at the C-terminus.
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Source : HEK293
Species : Human
Tag : hFc
Form : Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Bio-activity : Measured by its binding ability in a functional ELISA. Immobilized FGF2 Protein, Human at 2 μg/mL (100 μL/well) can bind Glypican 3 Protein, Human, Recombinant (495S/A, hFc Tag), the EC50 of Glypican 3 is 0.75-1.1 μg/mL.
Molecular Mass : The recombinant human GPC3 consists of 764 amino acids and predicts a molecular mass of 86.46 kDa.
Protein length : Met1-Ser550
Endotoxin : < 1.0 EU per μg protein as determined by the LAL method.
Purity : > 95 % as determined by SDS-PAGE.
Storage : Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
Gene Name : GPC3 glypican 3 [ Homo sapiens ]
Official Symbol : GPC3
Synonyms : GPC3; glypican 3; SDYS; glypican-3; DGSX; glypican proteoglycan 3; OCI 5; SGB; SGBS; SGBS1; secreted glypican-3; intestinal protein OCI-5; heparan sulphate proteoglycan; MXR7; OCI-5; GTR2-2;
Gene ID : 2719
mRNA Refseq : NM_001164617
Protein Refseq : NP_001158089
MIM : 300037
UniProt ID : P51654

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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08/12/2020

    The GPC3 protein offered by the manufacturer is of exceptional quality, making it an ideal choice to fulfill my experimental needs.

    09/18/2019

      The technical support provided by the manufacturer is invaluable in overcoming experimental hurdles and optimizing the utilization of GPC3 protein.

      04/10/2019

        Another crucial aspect where the manufacturer excels is their efficient supply chain management.

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