| Description : |
This gene encodes a member of the insulin-like growth factor (IGF) family of proteins that promote growth and development during fetal and postnatal life. This gene is predominantly expressed in the liver and the encoded protein undergoes proteolytic processing to generate a disulfide-linked mature polypeptide. Transgenic disruption of this gene in mice results in reduced postnatal survival and severe growth retardation. Mice lacking the encoded protein exhibit generalized organ hypoplasia including underdevelopment of the central nervous system and developmental defects in bone, muscle and reproductive systems. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate mature protein. |
| Source : |
E. coli |
| Species : |
Mouse |
| Bio-activity : |
The ED50 is 3-15 ng/ml, corresponding to a specific activity of 0.6-3.3 x 105 units/mg, determined by the dose dependent stimulation of MCF-7 cell proliferation. |
| Molecular Mass : |
7.8 kDa |
| Endotoxin : |
Less than 0.01 ng per µg protein as determined by the LAL method |
| Purity : |
>95%, as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Unopened vial can be stored between 2 centigrade and 8 centigrade for up to 2 weeks, at -20 centigrade for up to 6 months, or at -70 centigrade or below until the expiration date. Aliquots can be stored between 2 centigrade and 8 centigrade for up to one week and stored at -20 centigrade or colder for up to 3 months. Avoid repeated freeze/thaw cycles. |
| Storage : |
Store at -80 centigrade. |
| Concentration : |
Protein formulated and vialed at >50 ug/mL in specified buffer |
| Storage Buffer : |
1 x PBS |
