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Active Recombinant Mouse Il10 Protein (161 aa)

Cat.No. : Il10-029I
Product Overview : Recombinant Mouse Il10 Protein without tag was expressed in E. coli.
  • Specification
  • Gene Information
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Description : IL-10 is an immunosuppressive cytokine produced by a variety of mammalian cell types including macrophages, monocytes, T cells, B cells and keratinocytes. IL-10 inhibits the expression of proinflammatory cytokines such as IL-1 and TNFα. Like IL-4, IL-10 enhances humoral immune responses and attenuates cell-mediated immune reactions. Human IL-10 active on murine cells, but murine IL-10 is inactive on human cells.
Source : E. coli
Species : Mouse
Form : Sterile Filtered White lyophilized (freeze-dried) powder.
Bio-activity : Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent co-stimulation (with IL-4) of the proliferation of murine MC/9 cells was found to be < 2 ng/mL, corresponding to a specific activity of > 5 × 10^5 units/mg.
Molecular Mass : Approximately 18.7 kDa, a single non-glycosylated polypeptide chain containing 161 amino acids
Protein Length : 161
AA Sequence : MSRGQYSREDNNCTHFPVGQSHMLL ELRTAFSQVKTFFQTKDQLDNILLT DSLMQDFKGYLGCQALSEMIQFYLV EVMPQAEKHGPEIKEHLNSLGQKLK TLRMRLRRCHRFLPCENKSKAVEQV KSDFNKLEDQGVYKAMNEFDIFINC IEAYMMIKMKS
Endotoxin : Less than 1 EU/mg of rMuIL-10 as determined by LAL method.
Purity : >97% by SDS-PAGE and HPLC analyses.
Storage : This lyophilized preparation is stable at 2-8 centigrade, but should be kept at -20 centigrade for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 centigrade. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 to -70 centigrade. Avoid repeated freeze/thaw cycles.
Storage Buffer : Lyophilized from a 0.2mm filtered concentrated solution in PBS, pH 7.4.
Reconstitution : We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at < -20 centigrade. Further dilutions should be made in appropriate buffered solutions.
Gene Name : Il10 interleukin 10 [ Mus musculus ]
Official Symbol : Il10
Synonyms : IL10; interleukin 10; interleukin-10; cytokine synthesis inhibitory factor; CSIF; Il-10;
Gene ID : 16153
mRNA Refseq : NM_010548
Protein Refseq : NP_034678
UniProt ID : P18893

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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What state-of-the-art experimental strategies, including advanced imaging techniques, are implemented to meticulously assess the in vivo efficacy of IL-10-based therapies across a spectrum of inflammatory disorders? 02/12/2022

Cutting-edge imaging techniques, alongside in vivo disease models, allow for precise assessment of therapeutic effects and tissue responses to IL-10-based treatments.

How are advanced methods like single-cell RNA sequencing and flow cytometry harnessed to investigate the immunomodulatory impact of IL-10 across distinct immune cell subtypes? 02/10/2022

Advanced technologies, including single-cell RNA sequencing and flow cytometry, enable a granular analysis of IL-10's impact on diverse immune cell subpopulations.

Could you elaborate on the intricate experimental methodologies applied to unravel the influence of genetic polymorphisms within IL-10 and its receptor on disease susceptibility? 10/26/2021

Genotyping studies and genetic association analyses help correlate variations in IL-10 and its receptor with disease susceptibility across diverse populations.

How do researchers leverage advanced immunomonitoring tools to comprehensively evaluate the safety profiles of IL-10-based interventions in various clinical trial phases? 05/25/2021

Advanced immunomonitoring tools, including multiplex cytokine assays and immune cell phenotyping, provide comprehensive safety evaluations of IL-10 interventions in clinical trials.

How do researchers employ in vitro models to elucidate the mechanisms underlying IL-10's anti-inflammatory effects on immune cells? 12/03/2020

In vitro models involve exposing immune cells to IL-10 and assessing changes in cytokine secretion and cell activation markers to uncover its anti-inflammatory mechanisms.

What sophisticated experimental techniques are utilized to dissect the intricate signaling pathways downstream of IL-10 activation? 08/26/2020

State-of-the-art techniques such as phosphoproteomics, mass spectrometry, and protein-protein interaction assays provide insights into the intricate signaling cascades activated by IL-10.

Could you explain the multifaceted experimental strategies employed to determine the optimal dosage and administration routes for IL-10 in diverse animal models? 03/24/2019

Dose-response studies in animal models coupled with outcome measures like cytokine profiles and immune cell phenotypes help determine the optimal IL-10 dosage and administration routes.

What innovative experimental designs are employed to explore potential synergies between IL-10 and other cytokines, unraveling complex interplay in immune regulation? 11/23/2018

Experimental designs encompass co-stimulation experiments, coupled with functional assays and high-dimensional analysis, to elucidate synergistic effects involving IL-10 and other cytokines.

Can you elaborate on the comprehensive approaches scientists use to assess the tissue-specific expression patterns of IL-10 receptors in both normal and pathological conditions? 11/05/2018

Techniques like RT-PCR, immunohistochemistry, and spatial transcriptomics are employed to characterize IL-10 receptor expression patterns in specific tissues and disease contexts.

How are cutting-edge techniques such as CRISPR/Cas9 gene editing utilized to dissect the regulatory roles of IL-10 in complex autoimmune disease models? 02/22/2018

CRISPR/Cas9 gene editing is used to create knockout or overexpression models, combined with functional assays, to decipher IL-10's regulatory roles in autoimmune disease pathways.

Customer Reviews (3)

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Reviews
09/27/2022

    Effective in anti-inflammatory assays.

    01/09/2019

      High purity levels.

      04/15/2017

        Stable under long-term storage.

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