Active Recombinant Chicken Interleukin 10

• Cat.No.: IL10-246C
• Product Overview: Recombinant Chicken IL-10 was expressed inYeast. MW= 18.3 kDa (154 aa).

Specification

• Species: Chicken
• Source: Yeast
• Tag: Non
• Description: Interleukin-10 is produced by murine T-cells following their stimulation by lectins. The main source for B-cell-derived Interleukin-10 in mice is Ly-1 B-cells that express CD5 and CD11. Murine keratinocytes also produce IL10.
• Formulation/ Reconstitution: Lyophilized. Reconstitute with sterile phosphate-buffered saline containing at least 0.1% carrier protein.
• Purification: Ion-exchange chromatography.
• Purity: >95% as visualized by SDS-PAGE analysis.
• Amino Acid Sequence: LEPTCLHFSE LLPARLRELR VKFEEIKDYF QSRDDELNIQ LLSSELLDKF KGTFGCQSVS EMLRFYTDEV LPRAMQTSTS HQQSMGDLGN MLLGLKATMR RCHRFFTCEK RSKAIKQIKE TFEKMDENGI YKAMGEFDIF INYIEEYLLM RRRK.
• Bioactivity: The biological activity of recombinant chicken IL-10 was determined by measuring IFN-gamma expression by RTPCR in Concanavalin A-stimulated chicken spleen lymphocytes. Results demonstrate decreased IFN-gamma gene expression, with the addition of recombinant chicken IL-10, in a dose-dependant manner, with doses starting at 2 ng/mL.
• Stability And Storage: Stable for up to twelve months from date of receipt at -20°C. Stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. Avoid repeated freeze/thaw cycles.

Gene Information

• Gene Name: IL10 interleukin 10 [ Gallus gallus ]
• Synonyms: interleukin 10; IL-10; IL10; interleukin-10
• Gene ID: 428264
• mRNA Refseq: NM_001004414.2
• Protein Refseq: NP_001004414.2
• UniProt ID: Q6A2H4
• Chromosome Location: 26
• Pathway: Cytokine-cytokine receptor interaction; Intestinal immune network for IgA production; Jak-STAT signaling pathway
• Function: cytokine activity; protein binding