Active Recombinant Chicken Interleukin 10
Cat.No. : | IL10-246C |
Product Overview : | Recombinant Chicken IL-10 was expressed inYeast. MW= 18.3 kDa (154 aa). |
- Specification
- Gene Information
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Cat. No. : | IL10-246C |
Description : | Interleukin-10 is produced by murine T-cells following their stimulation by lectins. The main source for B-cell-derived Interleukin-10 in mice is Ly-1 B-cells that express CD5 and CD11. Murine keratinocytes also produce IL10. |
Source : | Yeast. |
Formulation/ Reconstitution : | Lyophilized. Reconstitute with sterile phosphate-buffered saline containing at least 0.1% carrier protein. |
Purification : | Ion-exchange chromatography. |
Purity : | >95% as visualized by SDS-PAGE analysis. |
Amino Acid Sequence : | LEPTCLHFSE LLPARLRELR VKFEEIKDYF QSRDDELNIQ LLSSELLDKF KGTFGCQSVS EMLRFYTDEV LPRAMQTSTS HQQSMGDLGN MLLGLKATMR RCHRFFTCEK RSKAIKQIKE TFEKMDENGI YKAMGEFDIF INYIEEYLLM RRRK. |
Bioactivity : | The biological activity of recombinant chicken IL-10 was determined by measuring IFN-gamma expression by RTPCR in Concanavalin A-stimulated chicken spleen lymphocytes. Results demonstrate decreased IFN-gamma gene expression, with the addition of recombinant chicken IL-10, in a dose-dependant manner, with doses starting at 2 ng/mL. |
Stability And Storage : | Stable for up to twelve months from date of receipt at -20°C. Stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. Avoid repeated freeze/thaw cycles. |
Gene Name : | IL10 interleukin 10 [ Gallus gallus ] |
Synonyms : | interleukin 10; IL-10; IL10; interleukin-10 |
Gene ID : | 428264 |
mRNA Refseq : | NM_001004414.2 |
Protein Refseq : | NP_001004414.2 |
UniProt ID : | Q6A2H4 |
Chromosome Location : | 26 |
Pathway : | Cytokine-cytokine receptor interaction; Intestinal immune network for IgA production; Jak-STAT signaling pathway |
Function : | cytokine activity; protein binding |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionCutting-edge imaging techniques, alongside in vivo disease models, allow for precise assessment of therapeutic effects and tissue responses to IL-10-based treatments.
Advanced technologies, including single-cell RNA sequencing and flow cytometry, enable a granular analysis of IL-10's impact on diverse immune cell subpopulations.
Genotyping studies and genetic association analyses help correlate variations in IL-10 and its receptor with disease susceptibility across diverse populations.
Advanced immunomonitoring tools, including multiplex cytokine assays and immune cell phenotyping, provide comprehensive safety evaluations of IL-10 interventions in clinical trials.
In vitro models involve exposing immune cells to IL-10 and assessing changes in cytokine secretion and cell activation markers to uncover its anti-inflammatory mechanisms.
State-of-the-art techniques such as phosphoproteomics, mass spectrometry, and protein-protein interaction assays provide insights into the intricate signaling cascades activated by IL-10.
Dose-response studies in animal models coupled with outcome measures like cytokine profiles and immune cell phenotypes help determine the optimal IL-10 dosage and administration routes.
Experimental designs encompass co-stimulation experiments, coupled with functional assays and high-dimensional analysis, to elucidate synergistic effects involving IL-10 and other cytokines.
Techniques like RT-PCR, immunohistochemistry, and spatial transcriptomics are employed to characterize IL-10 receptor expression patterns in specific tissues and disease contexts.
CRISPR/Cas9 gene editing is used to create knockout or overexpression models, combined with functional assays, to decipher IL-10's regulatory roles in autoimmune disease pathways.
Customer Reviews (3)
Write a reviewEffective in anti-inflammatory assays.
High purity levels.
Stable under long-term storage.
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