Active Recombinant Mouse Il33 Protein

• Cat.No.: Il33-5186M
• Product Overview: Mouse Il33 (Q8BVZ5, 109 a.a. - 266 a.a.) partial recombinant protein expressed in Escherichia coli.

Specification

• Description: Interleukin 33 (IL-33) is a protein that in humans is encoded by the IL33 gene. Interleukin 33 is a member of the IL-1 family that potently drives production of T helper-2 (Th2)-associated cytokines (e.g., IL-4). IL33 is a ligand for ST2 (IL1RL1), an IL-1 family receptor that is highly expressed on Th2 cells, mast cells and group 2 innate lymphocytes. IL-33 is expressed by a wide variety of cell types, including fibroblasts, mast cells, dendritic cells, macrophages, osteoblasts, endothelial cells, and epithelial cells.
• Source: E. coli
• Species: Mouse
• Form: Lyophilized
• Bio-activity: The ED50 was determined by the dose-dependent proliferation of murine D10S cells is ≤ 0.3 ng/mL.
• Molecular Mass: 18 kDa
• AA Sequence: SIQGTSLLTQSPASLSTYNDQSVSF VLENGCYVINVDDSGKDQEQDQVLL RYYESPCPASQSGDGVDGKKLMVNM SPIKDTDIWLHANDKDYSVELQRGD VSPPEQAFFVLHKKSSDFVSFECKN LPGTYIGVKDNQLALVEEKDESCNN IMFKLSKI
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/μg (1EU/μg).
• Purity: > 90% by SDS-PAGE and HPLC
• Applications: Functional Study
  SDS-PAGE
• Storage: Store at -20 centigrade on dry atmosphere for 2 years. After reconstitution with deionized water, store at 4 centigrade for 1 month or store at -20 centigrade for 6 months. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: Lyophilized from PBS, pH 7.5

Gene Information

• Gene Name: Il33 interleukin 33 [ Mus musculus ]
• Official Symbol: Il33
• Synonyms: IL33; interleukin 33; interleukin-33; nuclear factor from high endothelial venules; Il-33; Il1f11; NF-HEV; 9230117N10Rik
• Gene ID: 77125
• mRNA Refseq: NM_001164724
• Protein Refseq: NP_001158196

Reviews

10/16/2021

Good solubility and is easy to operate and purify.

05/03/2021

Effectively mimics the function of the target protein in vitro.

03/18/2020

IL33 has a wide range of applications in the field of scientific research and is suitable for a variety of experimental design and research purposes.

Q&As

Which immune cells can recombinant IL33 be used to detect?

It can be used to detect immune cells such as macrophages and dendritic cells.

In what research fields can recombinant IL33 be applied?

This protein applied in immunology, drug research, life science and other fields.

What is the action mechanism of IL33?

Regulates the activity of immune cells by binding to receptors to stimulate the production of inflammatory factors such as interferon and TNF-α.

Besides treating asthma and hay fever, what other diseases can be treated with IL33?

IL33 can also be used to treat a variety of inflammatory diseases, such as inflammatory bowel disease, psoriasis and so on.

How to prepare IL33?

The preparation of IL33 requires first cloning the IL33 gene into the expression vector, and then the expression vector is expressed and purified in the cell culture medium.

How long does it take to treat asthma with IL33?

Treating asthma with recombinant IL33 takes several weeks to several months to be effective, depending on the severity of the condition and the individual patient.