Recombinant Mouse L3mbtl1 Protein, Myc/DDK-tagged

• Cat.No.: L3mbtl1-3743M
• Product Overview: Purified recombinant protein of mouse full-length L3MBTL1 histone methyl-lysine binding protein (L3mbtl1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Polycomb group (PcG) protein that specifically recognizes and binds mono- and dimethyllysine residues on target proteins, therey acting as a 'reader' of a network of post-translational modifications. PcG proteins maintain the transcriptionally repressive state of genes: acts as a chromatin compaction factor by recognizing and binding mono- and dimethylated histone H1b/H1-4 at 'Lys-26' (H1bK26me1 and H1bK26me2) and histone H4 at 'Lys-20' (H4K20me1 and H4K20me2), leading to condense chromatin and repress transcription. Recognizes and binds p53/TP53 monomethylated at 'Lys-382', leading to repress p53/TP53-target genes. Also recognizes and binds RB1/RB monomethylated at 'Lys-860'. Participates in the ETV6-mediated repression. Probably plays a role in cell proliferation. Overexpression induces multinucleated cells, suggesting that it is required to accomplish normal mitosis.
• Molecular Mass: 92.2 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: L3mbtl1 L3MBTL1 histone methyl-lysine binding protein [ Mus musculus (house mouse) ]
• Official Symbol: L3mbtl1
• Synonyms: L3MBTL1; l(3)mbt-like (Drosophila); lethal(3)malignant brain tumor-like protein 1; H-l(3)mbt protein; L(3)mbt protein homolog; L3mbtl; mKIAA0681; C630004G01; KIAA0681
• Gene ID: 241764
• mRNA Refseq: NM_001081338
• Protein Refseq: NP_001074807
• UniProt ID: A2A5N8