Recombinant Mouse Mdm2 Protein, His-tagged

• Cat.No.: Mdm2-1771M
• Product Overview: Recombinant Mouse Mdm2 Protein (Met1-Asn310) with N-His tag was expressed in E. coli.

Specification

• Description: Enables p53 binding activity and ubiquitin protein ligase activity. Involved in apoptotic process and positive regulation of muscle cell differentiation. Acts upstream of or within several processes, including blood vessel remodeling; circulatory system development; and traversing start control point of mitotic cell cycle. Located in cytoplasm and nucleolus. Is expressed in several structures, including alimentary system; embryo ectoderm; genitourinary system; respiratory system; and sensory organ. Human ortholog(s) of this gene implicated in chronic myeloid leukemia; endocrine gland cancer (multiple); glioblastoma; ichthyosis; and sarcoma. Orthologous to human MDM2 (MDM2 proto-oncogene).
• Source: E. coli
• Species: Mouse
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 38.2 kDa
• Protein length: Met1-Asn310
• Purity: > 97%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Mdm2 transformed mouse 3T3 cell double minute 2 [ Mus musculus (house mouse) ]
• Official Symbol: Mdm2
• Synonyms: MDM2; transformed mouse 3T3 cell double minute 2; E3 ubiquitin-protein ligase Mdm2; oncoprotein Mdm2; double minute 2 protein; p53-binding protein Mdm2; Mdm-2; AA415488; 1700007J15Rik
• Gene ID: 17246
• mRNA Refseq: NM_010786
• Protein Refseq: NP_034916
• UniProt ID: P23804

Reviews

12/24/2021

Validated in multiple assays.

07/14/2019

Great for cell culture.

04/05/2019

Consistent performance, highly reproducible.

Q&As

How does MDM2 regulate the tumor suppressor protein p53?

MDM2 binds directly to p53, inhibiting its transcriptional activity. Additionally, MDM2 ubiquitinates p53, targeting it for proteasomal degradation, thus regulating p53 levels in the cell.

What is the significance of the MDM2-p53 feedback loop in cellular regulation?

The MDM2-p53 feedback loop is crucial for cellular homeostasis. When DNA damage occurs, p53 levels rise, leading to cell cycle arrest or apoptosis. Elevated p53 can induce MDM2 expression, which in turn downregulates p53, ensuring that p53 activity is transient.

How do amplifications or overexpression of MDM2 contribute to cancer progression?

Amplifications or overexpression of MDM2 can lead to reduced p53 activity, even in the presence of DNA damage or other stress signals, potentially contributing to uncontrolled cell proliferation and tumorigenesis.

Are there any known inhibitors targeting MDM2, and what are their therapeutic implications?

Several small molecule inhibitors, like Nutlin-3 and RG7112, target MDM2 to prevent its interaction with p53, thereby stabilizing and activating p53. These inhibitors hold therapeutic potential, especially in tumors with wild-type p53 but overexpressed MDM2.

How does the interaction between MDM2 and other proteins influence cell cycle and apoptosis?

Besides p53, MDM2 interacts with other proteins, including E2F1 and RB, influencing cell cycle progression and apoptosis. The balance of these interactions can determine cell fate.

What is the primary function of the MDM2 gene and its encoded protein?

The MDM2 gene encodes a nuclear-localized E3 ubiquitin ligase. Its primary function is to regulate the p53 tumor suppressor protein's stability and activity.

What are the potential therapeutic strategies for targeting MDM2 in cancer treatment?

Therapeutic strategies targeting MDM2 include the use of MDM2 inhibitors to reactivate p53, combination therapies with DNA-damaging agents, and strategies to disrupt MDM2's interaction with other proteins to modulate cell cycle and apoptosis.