Recombinant Mouse S100a5 Protein, His-tagged

• Cat.No.: S100a5-7325M
• Product Overview: Recombinant mouse S100A5 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specification

• Description: Binds calcium, zinc and copper. One subunit can simultaneously bind 2 calcium ions or 2 copper ions plus 1 zinc ion. Calcium and copper ions compete for the same binding sites.
• Source: E. coli
• Species: Mouse
• Tag: His
• Form: Liquid
• Molecular Mass: 13.4 kDa
• Protein length: 1-93
• AA Sequence: MGSSHHHHHHSSGLVPRGSHMGSHM ETPLEKALTTMVTTFHKYSGREGSK LTLSRKELKELIKTELSLAEKMKES SIDNLMKSLDKNSDQEIDFKEYSVF LTTLCMAYNDFFLEDNK
• Purity: > 95 % by SDS-PAGE
• Stability: Shelf life: one year from despatch.
• Storage: Store undiluted at 2-8 centigrade for one week or (in aliquots) at -20 to -80 centigrade for longer. Avoid repeated freezing and thawing.
• Concentration: 1.0 mg/mL (determined by Bradford assay)
• Storage Buffer: 20 mM Tris-HCl buffer, pH 8.0, 40 % glycerol, 3 mM DTT, 200 mM NaCl

Gene Information

• Gene Name: S100a5 S100 calcium binding protein A5 [ Mus musculus (house mouse) ]
• Official Symbol: S100a5
• Synonyms: S100a5; S100 calcium binding protein A5; S100D; S100D9; protein S100-A5
• Gene ID: 20199
• mRNA Refseq: NM_011312
• Protein Refseq: NP_035442
• UniProt ID: P63084

Reviews

05/07/2020

Reliable antibody validation, vital for our experiments.

06/24/2019

Efficient peptide synthesis, accelerates our peptide research.

04/25/2018

Accurate mass spectrometry analysis, crucial for proteomics studies.

Q&As

How does S100A5 interact with calcium ions and what are the implications of this interaction for cellular signaling?

S100A5 binds calcium ions, affecting cellular signaling pathways and calcium-dependent processes.

What are the effects of oxidative stress on the function and regulation of S100A5?

Oxidative stress impacts S100A5 function, affecting its regulatory role in cellular processes.

What role does S100A5 play in the regulation of inflammatory responses and immune cell function?

S100A5 is involved in regulating inflammation and immune cell function, influencing immune response.

How does S100A5 contribute to neuronal development and neuroplasticity?

S100A5 plays a role in neuronal development and neuroplasticity, affecting brain function and adaptation.

What is the impact of S100A5 expression on cancer development and progression?

S100A5 expression influences cancer development, potentially impacting tumor growth and metastasis.

How does S100A5 influence muscle contraction and relaxation mechanisms?

S100A5 affects muscle contraction and relaxation, playing a role in muscle function and coordination.

How do alterations in S100A5 expression impact cardiovascular health and disease?

Changes in S100A5 expression can influence cardiovascular health, impacting heart function and disease development.