Recombinant Mouse S100a5, His-tagged

• Cat.No.: S100a5-15850M
• Product Overview: Recombinant mouse S100A5 protein, fused to His tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Description: S100 calcium binding protein A5, also known as S100A5, is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A5 has a Ca2+ affinity 20- to 100-fold higher than the other S100 proteins studied under identical conditions. This protein also binds Zn2+ and Cu2+, and Cu2+ strongly which impairs the binding of Ca2+. It is expressed in very restricted regions of the adult brain.
• Source: E. coli
• Species: Mouse
• Tag: His
• Form: Liquid. In 20 mM Tris-HCl buffer, pH8.0, 40% glycerol, 3mM DTT, 200mM NaCl
• Molecular Mass: 13.4kDa (117aa), confirmed by MALDI-TOF
• Protein length: 1-93aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMETPLE KALTTMVTTF HKYSGREGSK LTLSRKELKE LIKTELSLAE KMKESSIDNL MKSLDKNSDQ EIDFKEYSVF LTTLCMAYND FFLEDNK
• Purity: >95% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term. For long term storage, aliquot and store at at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 1mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: S100a5 S100 calcium binding protein A5 [ Mus musculus ]
• Official Symbol: S100A5
• Synonyms: S100A5; S100 calcium binding protein A5; protein S100-A5; S100 calcium-binding protein A5; S100D9
• Gene ID: 20199
• mRNA Refseq: NM_011312
• Protein Refseq: NP_035442
• Function: calcium ion binding; copper ion binding; metal ion binding; protein homodimerization activity; zinc ion binding

Reviews

05/07/2020

Reliable antibody validation, vital for our experiments.

06/24/2019

Efficient peptide synthesis, accelerates our peptide research.

04/25/2018

Accurate mass spectrometry analysis, crucial for proteomics studies.

Q&As

How does S100A5 interact with calcium ions and what are the implications of this interaction for cellular signaling?

S100A5 binds calcium ions, affecting cellular signaling pathways and calcium-dependent processes.

What are the effects of oxidative stress on the function and regulation of S100A5?

Oxidative stress impacts S100A5 function, affecting its regulatory role in cellular processes.

What role does S100A5 play in the regulation of inflammatory responses and immune cell function?

S100A5 is involved in regulating inflammation and immune cell function, influencing immune response.

How does S100A5 contribute to neuronal development and neuroplasticity?

S100A5 plays a role in neuronal development and neuroplasticity, affecting brain function and adaptation.

What is the impact of S100A5 expression on cancer development and progression?

S100A5 expression influences cancer development, potentially impacting tumor growth and metastasis.

How does S100A5 influence muscle contraction and relaxation mechanisms?

S100A5 affects muscle contraction and relaxation, playing a role in muscle function and coordination.

How do alterations in S100A5 expression impact cardiovascular health and disease?

Changes in S100A5 expression can influence cardiovascular health, impacting heart function and disease development.