Recombinant Human S100A5 293 Cell Lysate

• Cat.No.: S100A5-2089HCL

Specification

• Description: Antigen standard for S100 calcium binding protein A5 (S100A5) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
• Tag: Non

Gene Information

• Gene Name: S100A5 S100 calcium binding protein A5 [ Homo sapiens ]
• Official Symbol: S100A5
• Synonyms: S100A5; S100 calcium binding protein A5; S100 calcium binding protein A5 , S100D; protein S100-A5; S100 calcium-binding protein A5; S100D
• Gene ID: 6276
• mRNA Refseq: NM_002962
• Protein Refseq: NP_002953
• MIM: 176991
• UniProt ID: P33763
• Chromosome Location: 1q21
• Function: calcium ion binding; copper ion binding; protein homodimerization activity; zinc ion binding

Reviews

05/07/2020

Reliable antibody validation, vital for our experiments.

06/24/2019

Efficient peptide synthesis, accelerates our peptide research.

04/25/2018

Accurate mass spectrometry analysis, crucial for proteomics studies.

Q&As

How does S100A5 interact with calcium ions and what are the implications of this interaction for cellular signaling?

S100A5 binds calcium ions, affecting cellular signaling pathways and calcium-dependent processes.

What are the effects of oxidative stress on the function and regulation of S100A5?

Oxidative stress impacts S100A5 function, affecting its regulatory role in cellular processes.

What role does S100A5 play in the regulation of inflammatory responses and immune cell function?

S100A5 is involved in regulating inflammation and immune cell function, influencing immune response.

How does S100A5 contribute to neuronal development and neuroplasticity?

S100A5 plays a role in neuronal development and neuroplasticity, affecting brain function and adaptation.

What is the impact of S100A5 expression on cancer development and progression?

S100A5 expression influences cancer development, potentially impacting tumor growth and metastasis.

How does S100A5 influence muscle contraction and relaxation mechanisms?

S100A5 affects muscle contraction and relaxation, playing a role in muscle function and coordination.

How do alterations in S100A5 expression impact cardiovascular health and disease?

Changes in S100A5 expression can influence cardiovascular health, impacting heart function and disease development.