|Description:||The SMYD3 Homogeneous Assay Kit is designed to measure SMYD3 activity for screening and profiling applications. SMYD3 is a histone methyltransferase that promotes diand trimethylation of lysine4 of histone H3. It also exhibits methylation activity toward a lysine residue of MAPK/ERK Kinase Kinase 2 (MAP3K2). The key to the SMYD3 Homogeneous Assay Kit is a highly specific antibody that recognizes methylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing SMYD3 enzyme is incubated with the substrate for three hours. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.|
|Applications:||Great for studying enzyme kinetics and HTS applications.|
|Storage:||12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.|
|Kit Components:||SMYD3/HSP90: 20 µg; -80°C 100 µM S-adenosylmethionine: 2 x 250 μl; -80°CPrimary antibody 15: 5 μl; -80°CSMYD3 substrate: 200 μl; -80°C 4x SMYD3 assay Buffer 2: 3 x 1 ml; -20°C 4x Detection buffer 3: 2 x 1 ml; -20°C|
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